牛心脏线粒体中依赖环磷酸腺苷的蛋白质磷酸化作用

cAMP-dependent protein phosphorylation in mitochondria of bovine heart.

作者信息

Technikova-Dobrova Z, Sardanelli A M, Stanca M R, Papa S

机构信息

Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.

出版信息

FEBS Lett. 1994 Aug 22;350(2-3):187-91. doi: 10.1016/0014-5793(94)00760-8.

DOI:10.1016/0014-5793(94)00760-8

PMID:8070561

Abstract

A study is presented of the cAMP-dependent phosphorylation in bovine heart mitochondria of three proteins of 42, 16 and 6.5 kDa associated to the inner membrane. These proteins are also phosphorylated by the cytosolic cAMP-dependent protein kinase and by the purified catalytic subunit of this enzyme. In the cytosol, proteins of 16 and 6.5 kDa are phosphorylated by the cAMP-dependent kinase. It is possible that cytosolic and mitochondrial cAMP-dependent kinases phosphorylate the same proteins in the two compartments.

摘要

本文介绍了一项关于牛心脏线粒体中与内膜相关的三种蛋白质（42kDa、16kDa和6.5kDa）的cAMP依赖性磷酸化的研究。这些蛋白质也可被胞质cAMP依赖性蛋白激酶和该酶的纯化催化亚基磷酸化。在胞质溶胶中，16kDa和6.5kDa的蛋白质可被cAMP依赖性激酶磷酸化。胞质和线粒体cAMP依赖性激酶有可能在两个区室中磷酸化相同的蛋白质。