Segura-Aguilar J, Cremades A, Llombart-Bosch A, Monsalve E, Ernster L, Romero F J
Department of Physiology, School of Medicine and Dentistry, University of Valencia, Spain.
Carcinogenesis. 1994 Aug;15(8):1631-6. doi: 10.1093/carcin/15.8.1631.
We have studied the biochemical and immunohistochemical changes of DT-diaphorase in diethylstilbestrol (DES)-induced hamster kidney tumours and human biopsies from normal kidneys and renal clear cell carcinoma. The activities of primary and secondary antioxidants in these hamster and human tissues are also reported. DT-diaphorase is decreased in the different subcellular fractions of hamster and human tissues. In hamster kidney the activities of the one-electron quinone reductases show a nearly two-fold increase. Immunohistochemical findings confirm the decrease in DT-diaphorase in hamster and human tissues. This image is of special interest in the case of nephroblastoma (Wilms' tumour), since it has been proposed that the DES-induced tumour is a 'nephroblastoma-like' one. Primary anti oxidant enzymatic activities, i.e. superoxide dismutase and glutathione peroxidase, are increased in hamster kidney bearing DES-induced tumours and decreased in human renal clear cell carcinoma. Glutathione disulphide reductase is decreased in hamster and human tumours. The role of these enzymatic activities in the carcinogenic process is also discussed.
我们研究了己烯雌酚（DES）诱导的仓鼠肾肿瘤以及取自正常肾脏和肾透明细胞癌的人体活检组织中DT-黄递酶的生化和免疫组化变化。还报告了这些仓鼠和人体组织中一级和二级抗氧化剂的活性。在仓鼠和人体组织的不同亚细胞组分中，DT-黄递酶含量降低。在仓鼠肾脏中，单电子醌还原酶的活性增加了近两倍。免疫组化结果证实了仓鼠和人体组织中DT-黄递酶含量降低。在肾母细胞瘤（威尔姆斯瘤）的情况下，这一现象特别值得关注，因为有人提出DES诱导的肿瘤是一种“类肾母细胞瘤”。在携带DES诱导肿瘤的仓鼠肾脏中，一级抗氧化酶活性即超氧化物歧化酶和谷胱甘肽过氧化物酶增加，而在人类肾透明细胞癌中则降低。在仓鼠和人类肿瘤中，谷胱甘肽二硫化物还原酶减少。还讨论了这些酶活性在致癌过程中的作用。
