Dreher Z, Tout S, Stone J
Department of Anatomy and Histology, University of Sydney, NSW, Australia.
Glia. 1994 May;11(1):57-63. doi: 10.1002/glia.440110108.
We have studied the behaviour of living, process-bearing astrocytes in vitro, observing groups of cells at daily intervals for up to 7 days. Each cell initially formed two processes, appearing bipolar in shape, and with further time in culture, grew additional processes and appeared stellate. As their processes grew, the interactions between astrocytes underwent characteristic changes. While bipolar, the cells appeared to avoid making contact, lying parallel to each other. As they became stellate, the astrocytes made extensive contact with neighbours, gradually forming extended, contacting networks in which their somas were regularly spaced (as previously described). The interactions which led to the establishing of such arrays were also evident. If two cells were initially close or adjacent, they extended short processes to contact each other; then, as their processes grew, their somas moved apart, until they were separated by 60-120 microns. If two cells were initially well separated, each directed processes towards the other until contact was made, often with striking precision, and their somas then moved together, until they were separated by 60-120 microns. These behaviours of contact, separation, and approach caused astrocytes to form clusters, within which their somas appeared regularly spaced, and may represent the interactions which occur among astrocytes during normal development to produce the regularly spaced arrays of astrocytes described in earlier studies of intact central nervous tissue.
我们研究了体外培养的、具有突起的活星形胶质细胞的行为，每隔一天观察细胞群，持续观察长达7天。每个细胞最初形成两个突起，呈双极形状，随着培养时间的延长，会生长出更多突起并呈星形。随着它们的突起生长，星形胶质细胞之间的相互作用发生了特征性变化。在呈双极状态时，细胞似乎避免相互接触，彼此平行排列。当它们变成星形时，星形胶质细胞与相邻细胞广泛接触，逐渐形成延伸的、相互接触的网络，其中它们的胞体规则排列（如先前所述）。导致形成这种排列的相互作用也很明显。如果两个细胞最初靠得很近或相邻，它们会伸出短突起相互接触；然后，随着它们的突起生长，它们的胞体分开，直到相距60 - 120微米。如果两个细胞最初相距很远，每个细胞都会向另一个细胞伸出突起，直到接触，通常非常精确，然后它们的胞体一起移动，直到相距60 - 120微米。这些接触、分离和靠近的行为使星形胶质细胞形成簇，其中它们的胞体呈规则排列，可能代表了在正常发育过程中星形胶质细胞之间发生的相互作用，从而产生了早期对完整中枢神经组织研究中所描述的规则排列的星形胶质细胞阵列。
