Schell H, Kiesewetter F, Hornstein O P
Dermatologische Universitäts-Klinik, Erlangen.
Hautarzt. 1994 Jun;45(6):360-3. doi: 10.1007/s001050050083.
Androgenetic hair loss is the most frequent reason for the topical application of hair-growth-promoting agents. Such preparations should arrest or even reverse androgen-induced hair follicle regression as well as prolonging the hair cycles, especially of the shortened anagen phase, and thus protect from increased hair loss. True evidence of drug effects on hair growth is problematic, since trichograms, the method chiefly applied by the manufacturers, fail to reveal every factor involved in the follicular activity, especially the duration of anagen stage. For example, an increase in the anagen rate does not always reflect a lengthening of the anagen stage, but may also be due to shortened hair cycles. Accordingly, drug effects on hair growth should be investigated by methods that analyse the cell cycle kinetics. For this approach DNA-flowcytometry of the outer root sheath in plucked anagen hairs and of complete anagen hair bulbs taken by micropreparative techniques from scalp biopsies offers a reproducible method for quick and reliable evaluation of hair growth.
雄激素性脱发是局部应用促毛发生长剂的最常见原因。此类制剂应阻止甚至逆转雄激素诱导的毛囊退化，并延长毛发周期，尤其是缩短的生长期，从而防止脱发增加。药物对毛发生长的实际效果难以确定，因为制造商主要采用的毛发取样检查法无法揭示毛囊活动涉及的每个因素，尤其是生长期的持续时间。例如，生长期速率的增加并不总是反映生长期的延长，也可能是由于毛发周期缩短所致。因此，应通过分析细胞周期动力学的方法来研究药物对毛发生长的影响。对于这种方法，对拔下的生长期毛发外根鞘以及通过显微制备技术从头皮活检中获取的完整生长期毛球进行DNA流式细胞术，为快速可靠地评估毛发生长提供了一种可重复的方法。
