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大肠杆菌ilvIH启动子区域Lrp结合的体内足迹分析。

In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli.

作者信息

Marasco R, Varcamonti M, La Cara F, Ricca E, De Felice M, Sacco M

机构信息

Dipartimento di Fisiologia Generale ed Ambientale, Università Federico II, Naples, Italy.

出版信息

J Bacteriol. 1994 Sep;176(17):5197-201. doi: 10.1128/jb.176.17.5197-5201.1994.

DOI:10.1128/jb.176.17.5197-5201.1994
PMID:8071194
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196701/
Abstract

An in vivo footprinting analysis of the ilvIH regulatory region of Escherichia coli showed that the transcription activator Lrp binds to six sites, scattered over 250 bp upstream of the transcriptional start point. When Lrp-mediated activation was impaired by the presence of exogenous leucine, only one promoter-distal site (site 2) was partially protected by Lrp binding. Equilibrium dialysis experiments showed the formation of an Lrp-leucine complex in vitro. These results suggest that leucine negatively affects ilvIH transcription because its interaction with Lrp reduces the efficiency of binding of the regulatory protein to the promoter region.

摘要

对大肠杆菌ilvIH调控区进行的体内足迹分析表明，转录激活因子Lrp结合于六个位点，这些位点分散在转录起始点上游250 bp范围内。当外源亮氨酸的存在损害Lrp介导的激活作用时，只有一个启动子远端位点（位点2）因Lrp结合而受到部分保护。平衡透析实验表明，体外形成了Lrp-亮氨酸复合物。这些结果表明，亮氨酸对ilvIH转录产生负面影响，因为它与Lrp的相互作用降低了调节蛋白与启动子区域的结合效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047b/196701/85afaad0b470/jbacter00035-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047b/196701/7a30f9a349a9/jbacter00035-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047b/196701/85afaad0b470/jbacter00035-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047b/196701/7a30f9a349a9/jbacter00035-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047b/196701/85afaad0b470/jbacter00035-0016-a.jpg

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