大肠杆菌K-12的ilvHI操纵子的物理特性
Physical characterization of the ilvHI operon of Escherichia coli K-12.
作者信息
Squires C H, De Felice M, Wessler S R, Calvo J M
出版信息
J Bacteriol. 1981 Sep;147(3):797-804. doi: 10.1128/jb.147.3.797-804.1981.
Abstract
The ilvHI and leu genes of Escherichia coli K-12 are contained on a single 10.9-kilobase EcoRI fragment of deoxyribonucleic acid derived from the leu transducing phage lambda G4. Since the expression of all of these genes is controlled by leucine, we investigated whether they are part of single operon or whether they constitute separate but adjacent operons controlled from a common site. Both cloning and hybridization studies indicated that ilvHI and leu are distinct operons. They are transcribed in opposite directions and are separated by approximately 1,500 base pairs of deoxyribonucleic acid. Hybridization experiments showed that the expression of ilvHI is regulated chiefly at the level of transcription. The size of the ilvHI messenger ribonucleic acid is estimated to be 2,550 bases.
摘要
大肠杆菌K-12的ilvHI和leu基因位于来自亮氨酸转导噬菌体λG4的一条10.9千碱基的脱氧核糖核酸EcoRI片段上。由于所有这些基因的表达都受亮氨酸控制，我们研究了它们是单个操纵子的一部分，还是构成受共同位点控制的独立但相邻的操纵子。克隆和杂交研究均表明ilvHI和leu是不同的操纵子。它们以相反的方向转录，中间被大约1500个碱基对的脱氧核糖核酸隔开。杂交实验表明，ilvHI的表达主要在转录水平受到调控。ilvHI信使核糖核酸的大小估计为2550个碱基。
相似文献
1
Physical characterization of the ilvHI operon of Escherichia coli K-12.
J Bacteriol. 1981 Sep;147(3):797-804. doi: 10.1128/jb.147.3.797-804.1981.
2
IlvHI locus of Salmonella typhimurium.
J Bacteriol. 1983 Jun;154(3):1054-63. doi: 10.1128/jb.154.3.1054-1063.1983.
3
Acetohydroxy acid synthase isoenzymes of Escherichia coli K-12: a trans-acting regulatory locus of ilvHI gene expression.
Mol Gen Genet. 1981;181(4):491-6. doi: 10.1007/BF00428741.
4
Physical location of the ilvO determinant in Escherichia coli K-12 deoxyribonucleic acid.
J Bacteriol. 1980 May;142(2):547-55. doi: 10.1128/jb.142.2.547-555.1980.
5
Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.
J Bacteriol. 1977 Jul;131(1):214-23. doi: 10.1128/jb.131.1.214-223.1977.
6
Valine-resistant Escherichia coli K-12 strains with mutations in the ilvB operon.
J Bacteriol. 1981 Dec;148(3):998-1001. doi: 10.1128/jb.148.3.998-1001.1981.
7
Growth inhibition of Escherichia coli K-12 by L-valine: a consequence of a regulatory pattern.
Mol Gen Genet. 1977 Nov 4;156(1):1-7. doi: 10.1007/BF00272245.
8
DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12.
J Bacteriol. 1982 Jan;149(1):294-8. doi: 10.1128/jb.149.1.294-298.1982.
9
The ilvB locus of Escherichia coli K-12 is an operon encoding both subunits of acetohydroxyacid synthase I.
Nucleic Acids Res. 1985 Jun 11;13(11):3979-93. doi: 10.1093/nar/13.11.3979.
10
Relationship between messenger ribonucleic acid and enzyme levels specified by the leucine operon of Escherichia coli K-12.
J Bacteriol. 1977 Sep;131(3):997-1007. doi: 10.1128/jb.131.3.997-1007.1977.
引用本文的文献
1
Comparison of the genetic basis of biofilm formation between Typhimurium and .
Microb Genom. 2022 Nov;8(11). doi: 10.1099/mgen.0.000885.
2
Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides.
Plant Physiol. 1987 Dec;85(4):1110-7. doi: 10.1104/pp.85.4.1110.
3
Functional expression of plant acetolactate synthase genes in Escherichia coli.
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4179-83. doi: 10.1073/pnas.86.11.4179.
4
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
5
Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine.
J Bacteriol. 1993 Feb;175(4):1110-7. doi: 10.1128/jb.175.4.1110-1117.1993.
6
Subunit association in acetohydroxy acid synthase isozyme III.
J Bacteriol. 1993 Sep;175(17):5339-43. doi: 10.1128/jb.175.17.5339-5343.1993.
7
The H-NS protein modulates the activation of the ilvIH operon of Escherichia coli K12 by Lrp, the leucine regulatory protein.
Mol Gen Genet. 1994 Mar;242(6):736-43. doi: 10.1007/BF00283429.
8
In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli.
J Bacteriol. 1994 Sep;176(17):5197-201. doi: 10.1128/jb.176.17.5197-5201.1994.
9
The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli.
Microbiol Rev. 1994 Sep;58(3):466-90. doi: 10.1128/mr.58.3.466-490.1994.
10
Sequence determinants of DNA bending in the ilvlH promoter and regulatory region of Escherichia coli.
Nucleic Acids Res. 1994 Dec 25;22(25):5753-60. doi: 10.1093/nar/22.25.5753.
本文引用的文献
1
Molecular basis of valine resistance in Escherichia coli K-12.
Proc Natl Acad Sci U S A. 1981 Feb;78(2):922-5. doi: 10.1073/pnas.78.2.922.
2
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
The ilvG gene is expressed in Escherichia coli K-12.
Gene. 1980 Dec;12(1-2):165-70. doi: 10.1016/0378-1119(80)90028-1.
4
Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
Nucleic Acids Res. 1980 May 24;8(10):2255-74. doi: 10.1093/nar/8.10.2255.
5
Physical location of the ilvO determinant in Escherichia coli K-12 deoxyribonucleic acid.
J Bacteriol. 1980 May;142(2):547-55. doi: 10.1128/jb.142.2.547-555.1980.
6
Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants.
J Bacteriol. 1969 Mar;97(3):1272-82. doi: 10.1128/jb.97.3.1272-1282.1969.
7
Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12.
J Bacteriol. 1974 Dec;120(3):1068-77. doi: 10.1128/jb.120.3.1068-1077.1974.
8
Regulation of the pool size of valine in Escherichia coli K-12.
J Bacteriol. 1974 Dec;120(3):1058-67. doi: 10.1128/jb.120.3.1058-1067.1974.
9
Reaction of lac-specific ribonucleic acid from Escherichia coli with lac deoxyribonucleic acid.
J Biol Chem. 1974 Oct 25;249(20):6548-55.
10
Mutant of Escherichia coli K-12 missing acetolactate synthase activity.
J Bacteriol. 1974 Oct;120(1):536-8. doi: 10.1128/jb.120.1.536-538.1974.
Zotero 插件