来自铜绿假单胞菌的DD-羧肽酶和肽聚糖转肽酶。
DD-carboxypeptidase and peptidoglycan transpeptidase from Pseudomonas aeruginosa.
作者信息
Presslitz J E, Ray V A
出版信息
Antimicrob Agents Chemother. 1975 May;7(5):578-81. doi: 10.1128/AAC.7.5.578.
Abstract
Peptidoglycan transpeptidase and dd-carboxypeptidase have been detected in isolated membranes of Pseudomonas aeruginosa. Cephalosporins and penicillins fail to inhibit the transpeptidase at concentrations as high as 100 mug/ml. dd-Carboxypeptidase, on the other hand, is sensitive to inhibition by beta-lactam antibiotics. The presence of dimethyl sulfoxide in the reaction mixture results in a twofold stimulation of peptidoglycan formation, whereas dd-carboxypeptidase is inhibited approximately 30%. Maximum stimulation of transpeptidase occurs in the presence of both dimethyl sulfoxide and a beta-lactum antibiotic. This is in sharp contrast to the transpeptidase from Escherichia coli, which is sensitive to inhibition by penicillins and cephalosporins.
摘要
在铜绿假单胞菌的分离膜中已检测到肽聚糖转肽酶和D-羧肽酶。头孢菌素和青霉素在高达100μg/ml的浓度下无法抑制转肽酶。另一方面，D-羧肽酶对β-内酰胺抗生素的抑制敏感。反应混合物中存在二甲基亚砜会使肽聚糖形成受到两倍的刺激，而D-羧肽酶则被抑制约30%。转肽酶在二甲基亚砜和β-内酰胺抗生素同时存在的情况下受到最大刺激。这与大肠杆菌的转肽酶形成鲜明对比，后者对青霉素和头孢菌素的抑制敏感。
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本文引用的文献
1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
THE ENZYMATIC SYNTHESIS OF D-ALANYL-D-ALANINE. 3. ON THE INHIBITION OF D-ALANYL-D-ALANINE SYNTHETASE BY THE ANTIBIOTIC D-CYCLOSERINE.D-丙氨酰-D-丙氨酸的酶促合成。3. 抗生素D-环丝氨酸对D-丙氨酰-D-丙氨酸合成酶的抑制作用
Biochemistry. 1964 Apr;3:471-80. doi: 10.1021/bi00892a001.
3
The enzymatic addition of D-alanyl-D-alanine to a uridine nucleotide-peptide.将D-丙氨酰-D-丙氨酸酶促添加到尿苷核苷酸-肽上。
J Biol Chem. 1962 May;237:1601-4.
4
Enzymatic synthesis of cell wall mucopeptide in a particulate preparation of Escherichia coli.大肠杆菌颗粒制剂中细胞壁粘肽的酶促合成。
Biochem Biophys Res Commun. 1966 May 25;23(4):466-72. doi: 10.1016/0006-291x(66)90751-0.
5
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9
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