Matikainen T, Toppari J, Vihko K K, Huhtaniemi I
Department of Physiology, University of Turku, Finland.
J Endocrinol. 1994 Jun;141(3):449-57. doi: 10.1677/joe.0.1410449.
The mode of FSH actions within the testis was studied in immature hypophysectomized male rats by treatment with recombinant human FSH (recFSH, Org 32489). To elucidate the involvement of Leydig cells and androgens in the maintenance of spermatogenesis in FSH-treated hypophysectomized rats further, the recFSH treatment was given both alone and after destruction of Leydig cells with ethane-1,2-dimethane sulphonate (EDS). Three days after hypophysectomy (at 31 days of age) the rats were given one i.p. injection of vehicle or EDS and, 4 days later, they were implanted with osmotic minipumps releasing either 0.9% (w/v) NaCI or 1 IU recFSH/day. Recombinant FSH alone increased testicular weights 2.5-fold in 7 days (P < 0.01). The effect of FSH was similar in EDS-pretreated rats (P < 0.01). Testicular testosterone increased from 6.5 +/- 1.6 to 16.9 +/- 5.3 (S.E.M.) pmol/g tissue (P < 0.05) and serum testosterone from 0.12 +/- 0.02 to 0.22 +/- 0.03 nmol/l (P < 0.05) when the rats were treated with recFSH. EDS alone did not affect testicular testosterone but, when combined with recFSH, it totally abolished the stimulatory effect of FSH on testosterone. Testicular binding of 125I-labelled iodo human chorionic gonadotrophin (hCG) and 125I-labelled iodo recFSH was increased 2.5- and 2.1-fold respectively with recFSH treatment (P < 0.01). EDS, either alone or with FSH, abolished specific testicular hCG binding (P < 0.01), but had no effect on that of recFSH. However, FSH increased its own receptors only in animals not treated with EDS.(ABSTRACT TRUNCATED AT 250 WORDS)
通过用重组人促卵泡激素（recFSH，Org 32489）处理，在未成熟的垂体切除雄性大鼠中研究了促卵泡激素（FSH）在睾丸内的作用模式。为了进一步阐明睾丸间质细胞和雄激素在FSH处理的垂体切除大鼠精子发生维持中的作用，分别在单独给予recFSH处理以及用乙烷-1,2-二甲磺酸酯（EDS）破坏睾丸间质细胞后给予recFSH处理。垂体切除术后3天（31日龄），大鼠腹腔注射一次赋形剂或EDS，4天后，给它们植入释放0.9%（w/v）氯化钠或1 IU recFSH/天的渗透微型泵。单独使用重组FSH可使睾丸重量在7天内增加2.5倍（P < 0.01）。在EDS预处理的大鼠中，FSH的作用相似（P < 0.01）。当用recFSH处理大鼠时，睾丸睾酮从6.5±1.6升高至16.9±5.3（标准误）pmol/g组织（P < 0.05），血清睾酮从0.12±0.02升高至0.22±0.03 nmol/l（P < 0.05）。单独使用EDS不影响睾丸睾酮，但与recFSH联合使用时，它完全消除了FSH对睾酮的刺激作用。用recFSH处理后，125I标记的碘化人绒毛膜促性腺激素（hCG）和125I标记的碘化recFSH的睾丸结合分别增加了2.5倍和2.1倍（P < 0.01）。单独使用EDS或与FSH联合使用时，均可消除睾丸特异性hCG结合（P < 0.01），但对recFSH的结合无影响。然而，FSH仅在未用EDS处理的动物中增加其自身受体。（摘要截断于250字）
