DeFranzo A J, Morykwas M J, LaRosse J R, Jennings D A, Challa V, Argenta L C
Department of Plastic and Reconstructive Surgery, Wake Forest University Medical Center, Winston-Salem, North Carolina.
J Reconstr Microsurg. 1994 May;10(3):145-9; discussion 149-51. doi: 10.1055/s-2007-1006580.
This study examined the use of freeze-thawed muscle as a nerve graft material in Sprague-Dawley rats. In Group 1 (n = 4), the sciatic nerve was isolated and the incision immediately closed. In Group 2 (n = 4), a 5-mm segment of the nerve was removed and immediately replaced. In Group 3 (n = 50), a 5-mm segment of nerve was removed and muscle (2 x 2 x 5 mm, harvested from the gluteus, frozen in liquid nitrogen, and thawed in sterile water) was sutured in place. The short 5-mm segment of muscle provided an ideal situation for regeneration of myelinated nerve. Sciatic functional indices were comparable in all groups after 8 weeks and at 1 year. Histologic analysis showed comparable nerve regeneration in Groups 2 and 3 at 4 months. Group 3 grafts exhibited new axonal growth distal to the repaired gap at 4 weeks; myelinated fibers were present at 10 weeks. At 1 year, Group 3 nerves had almost the same axon count as contralateral nerves. However, myelin-sheath regeneration in the 5-mm muscle graft was incomplete, at 81 percent of normal, at 1 year.
本研究检测了冻融肌肉作为神经移植材料在Sprague-Dawley大鼠中的应用。第1组（n = 4），分离坐骨神经，切口立即闭合。第2组（n = 4），切除5毫米长的一段神经并立即进行替换。第3组（n = 50），切除5毫米长的一段神经，将肌肉（2×2×5毫米，取自臀肌，在液氮中冷冻并在无菌水中解冻）缝合到位。5毫米短段肌肉为有髓神经的再生提供了理想条件。8周后及1年时，所有组的坐骨神经功能指数相当。组织学分析显示，第2组和第3组在4个月时神经再生情况相当。第3组移植体在4周时在修复间隙远端出现新的轴突生长；10周时出现有髓纤维。1年时，第3组神经的轴突数量几乎与对侧神经相同。然而，5毫米肌肉移植体中的髓鞘再生不完全，1年时为正常的81%。
