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黑腹果蝇二倍体和三倍体中α-甘油磷酸脱氢酶的变体

Variants of alpha-glycerophosphate dehydrogenase in diploids and triploids of Drosophila melanogaster.

作者信息

Leibenguth F

出版信息

Biochem Genet. 1975 Apr;13(3-4):263-71. doi: 10.1007/BF00486021.

Abstract

On the basis of band staining intensities in electrophoretic runs of single flies homozygous and heterozygous for two alleles at the autosomal locus for GPDH, F allele activity is believed to be 8% lower than S allele activity. Indeed, the intensity distribution in the patterns of FSS and FFS triploid females shows that both are not equally expressed. On a per fly or live weight basis, females with two and three doses of the Gpdh gene show bands with equal staining intensity, thus exhibiting a dosage effect when GPDH activity is estimated on a per cell basis.

摘要

基于在常染色体GPDH位点上两个等位基因的纯合和杂合单只果蝇电泳跑胶中的条带染色强度,据信F等位基因活性比S等位基因活性低8%。实际上,FSS和FFS三倍体雌性的模式中的强度分布表明两者的表达并不相同。以每只果蝇或每单位活重计算,具有两个和三个剂量Gpdh基因的雌性显示出条带染色强度相同,因此在以每细胞为基础估算GPDH活性时表现出剂量效应。

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