Brown J A, Alcaraz A, Takahashi S, Persons D L, Lieber M M, Jenkins R B
Department of Urology, Mayo Clinic, Rochester, Minnesota 55905.
J Urol. 1994 Oct;152(4):1157-62. doi: 10.1016/s0022-5347(17)32527-2.
Fluorescent in situ hybridization using 12 chromosome enumeration probes (for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 17, 18, X and Y) was used to evaluate fresh tumor touch preparations from 40 randomly selected radical prostatectomy specimens. Of the tumors 16 (40%) contained chromosomal aneusomies. Chromosome 8 was aneusomic in 9 tumors (23%). Gain of chromosome 7 was observed in 8 tumors (20%). Chromosome 17 was aneusomic in 4 cases, and chromosomes 10, 11, 12, 18 and Y were each aneusomic twice. Loss of chromosome 9 was observed in 1 tumor. Chromosomes 4, 6, and X were never aneusomic. The percentage of monosomy 17 nuclei was 2 to 4 times the amount noted with the other autosomes for tumor and benign tissue. Computer analysis demonstrated that these signals contained twice the signal density and were significantly different (p < 0.0001) than the single diploid chromosome 17 signals. This result is consistent with homologous pairing of chromosome 17 in benign and neoplastic prostate tissue. Anomalies of chromosomes 8 and/or 7 were present in 14 of the 16 cases (88%) aneusomic by fluorescent in situ hybridization. High grade tumors were more likely to be aneuploid on fluorescent in situ hybridization (p < 0.001). Tumors with chromosome 8 aneusomies were of higher stage (p < 0.05). Fluorescent in situ hybridization is more sensitive than flow cytometry for the detection of aneusomy/aneuploidy. The prognostic relevance of these findings will require further investigation.
使用12种染色体计数探针（针对染色体4、6、7、8、9、10、11、12、17、18、X和Y）进行荧光原位杂交，以评估从40个随机选择的根治性前列腺切除标本中获取的新鲜肿瘤触片。在这些肿瘤中，16个（40%）含有染色体非整倍体。9个肿瘤（23%）中染色体8为非整倍体。在8个肿瘤（20%）中观察到染色体7的增加。4例中染色体17为非整倍体，染色体10、11、12、18和Y各有2例为非整倍体。在1个肿瘤中观察到染色体9的缺失。染色体4、6和X从未出现非整倍体。肿瘤和良性组织中17号染色体单体核的百分比是其他常染色体的2至4倍。计算机分析表明，这些信号的信号密度是单个二倍体染色体17信号的两倍，且差异显著（p < 0.0001）。这一结果与良性和肿瘤性前列腺组织中染色体17的同源配对一致。通过荧光原位杂交检测到的16例非整倍体病例中，14例（88%）存在染色体8和/或7的异常。高级别肿瘤在荧光原位杂交中更可能为非整倍体（p < 0.001）。存在染色体8非整倍体的肿瘤分期更高（p < 0.05）。荧光原位杂交在检测非整倍体/非整倍性方面比流式细胞术更敏感。这些发现的预后相关性需要进一步研究。
