Shibata T, Magari Y, Mizunaga S, Okabe E, Sumie A, Ishii T, Tomo T, Yasumori R, Nasu M
Second Department of Internal Medicine, Oita Medical University, Japan.
Nihon Jinzo Gakkai Shi. 1994 Jul;36(7):805-12.
In order to clarify the abnormalities of the coagulation and fibrinolysis system in patients with various renal diseases, we produced a new monoclonal antibody for FDP (fibrin/fibrinogen degradation product) D-dimer (D-D E72). We also established a new highly sensitive method of enzyme-linked immunosorbent assay (ELISA) for urinary FDP D-dimer using this monoclonal antibody. The urine from 110, patients with various renal diseases was investigated for the FDP D-dimer. The results are summarized as follows: 1) Urinary FDP D-dimer in normal subjects was 0.69 +/- 0.60 ng/ml. 2) The level of urinary FDP D-dimer in patients with primary nephrotic syndrome and in patients with chronic renal failure was significantly higher than that of normal subjects, whereas the urinary FDP D-dimer levels in patients with diabetes mellitus were higher than those of normal subjects. 3) In the CGN and NS groups there was a tendency for an increase in the level of urinary FDP D-dimer in more active forms of the disease. 4) A significant correlation between urinary FDP D-dimer and urinary protein in the CGN and NS groups was demonstrated. 5) In all of the renal diseases investigated in this study, the ratio of urinary FDP D-dimer to total FDP was less than 4%.
为阐明各种肾脏疾病患者凝血和纤溶系统的异常情况，我们制备了一种针对纤维蛋白/纤维蛋白原降解产物（FDP）D-二聚体（D-D E72）的新型单克隆抗体。我们还使用该单克隆抗体建立了一种新的高灵敏度酶联免疫吸附测定法（ELISA），用于检测尿FDP D-二聚体。对110例各种肾脏疾病患者的尿液进行了FDP D-二聚体检测。结果总结如下：1）正常受试者尿FDP D-二聚体为0.69±0.60 ng/ml。2）原发性肾病综合征患者和慢性肾衰竭患者尿FDP D-二聚体水平显著高于正常受试者，而糖尿病患者尿FDP D-二聚体水平高于正常受试者。3）在慢性肾小球肾炎（CGN）和肾病综合征（NS）组中，疾病活动度越高，尿FDP D-二聚体水平有升高趋势。4）CGN和NS组中，尿FDP D-二聚体与尿蛋白之间存在显著相关性。5）在本研究中所调查的所有肾脏疾病中，尿FDP D-二聚体占总FDP的比例均小于4%。
