Johnson M D, Contrino A, Contrino J, Maxwell K, Leonard G, Kreutzer D
Department of Surgery, University of Connecticut School of Medicine, Farmington, CT 06030-3105.
Laryngoscope. 1994 Sep;104(9):1143-9. doi: 10.1288/00005537-199409000-00016.
Recent studies have suggested that cytokines likely play a central role in the formation and maintenance of otitis media with effusion (OME). Currently, there is no immunologically defined animal model for the study of cytokines as they contribute to the formation of OME. In the present study, a murine model of OME, using eustachian tube blockage via an external surgical approach, was developed. The murine model temporal bone histology appears to mimic the histology found in chronic otitis media with effusion in humans. Additionally, using this murine model, interleukin-1 alpha (IL-1 alpha) expression was detected in the middle ear using standard immunohistochemical techniques. IL-1 alpha seemed localized to the epithelial lining of the middle ear as well as 5% to 10% of inflammatory cells. This model should provide the necessary tool to further study the immunologic aspects of OME.
近期研究表明，细胞因子可能在分泌性中耳炎（OME）的形成和维持过程中发挥核心作用。目前，尚无用于研究细胞因子在OME形成过程中作用的免疫明确动物模型。在本研究中，通过外部手术方法阻塞咽鼓管，建立了一种小鼠OME模型。该小鼠模型的颞骨组织学表现似乎与人类慢性分泌性中耳炎的组织学特征相似。此外，利用该小鼠模型，采用标准免疫组织化学技术在中耳检测到白细胞介素-1α（IL-1α）的表达。IL-1α似乎定位于中耳的上皮衬里以及5%至10%的炎性细胞中。该模型应为进一步研究OME的免疫学方面提供必要工具。
