Gulati A, Sharma A C, Burhop K E
Department of Pharmaceutics and Pharmacodynamics, University of Illinois at Chicago 60612.
Life Sci. 1994;55(10):827-37. doi: 10.1016/0024-3205(94)00566-4.
The effects of unmodified stroma-free hemoglobin (SFHb) and diaspirin cross-linked hemoglobin (DCLHb) on the regional blood circulation and systemic hemodynamics were studied in rats using a radioactive microsphere technique. SFHb and DCLHb increased mean arterial blood pressure without affecting heart rate. SFHb produced a 24.9% decrease in the cardiac output while DCLHb produced an 44.8% increase in the cardiac output. Stroke volume was decreased (-27.3%) by SFHb and increased (+36.4%) by DCLHb. Total peripheral resistance increased with both SFHb and DCLHb. DCLHb increased blood flow to the heart, spleen, stomach, small intestine and skin, and had no effect on blood flow to the brain, kidneys, liver, mesentery, pancreas, caecum, large intestine and musculo-skeletal system. In contrast, in animals infused with SFHb, blood flow decreased to the kidneys, liver and spleen, increased to the heart, small intestine and skin, and had no effect to the brain, caecum, large intestine and musculo-skeletal system. DCLHb had no effect on vascular resistance in any organ except for an increase in the musculo-skeletal system. In contrast, SFHb increased vascular resistance in the kidneys, liver, spleen, skin, mesentery and pancreas, and had no effect on vascular resistance in the musculo-skeletal system, brain, heart, stomach, small intestine, caecum and large intestine. SFHb had no effect on distribution of cardiac output to the brain, gastrointestinal tract (GIT), kidneys, skin, musculo-skeletal and portal system, while DCLHb significantly decreased the percent cardiac output to the musculo-skeletal system. DCLHb did not affect the distribution of cardiac output to the brain, GIT, kidneys, skin and portal system. SFHb and DCLHb increased the percent cardiac output to the heart. It is concluded that similar concentrations and doses of DCLHb and SFHb produce different effects on the regional blood circulation and systemic hemodynamics.
采用放射性微球技术，在大鼠身上研究了未修饰的无基质血红蛋白（SFHb）和双阿司匹林交联血红蛋白（DCLHb）对局部血液循环和全身血流动力学的影响。SFHb和DCLHb均可提高平均动脉血压，但不影响心率。SFHb使心输出量降低了24.9%，而DCLHb使心输出量增加了44.8%。每搏输出量在SFHb作用下降低了（-27.3%），在DCLHb作用下增加了（+36.4%）。SFHb和DCLHb均可使总外周阻力增加。DCLHb使心脏、脾脏、胃、小肠和皮肤的血流量增加，对脑、肾、肝、肠系膜、胰腺、盲肠、大肠和肌肉骨骼系统的血流量无影响。相比之下，在输注SFHb的动物中，肾、肝和脾脏的血流量减少，心脏、小肠和皮肤的血流量增加，对脑、盲肠、大肠和肌肉骨骼系统无影响。除肌肉骨骼系统阻力增加外，DCLHb对任何器官的血管阻力均无影响。相比之下，SFHb增加了肾、肝、脾、皮肤、肠系膜和胰腺的血管阻力，对肌肉骨骼系统、脑、心脏、胃、小肠、盲肠和大肠的血管阻力无影响。SFHb对心输出量在脑、胃肠道（GIT）、肾、皮肤、肌肉骨骼和门静脉系统的分布无影响，而DCLHb显著降低了心输出量在肌肉骨骼系统中的占比。DCLHb不影响心输出量在脑、GIT、肾、皮肤和门静脉系统的分布。SFHb和DCLHb均增加了心输出量在心脏中的占比。结论是，相似浓度和剂量的DCLHb和SFHb对局部血液循环和全身血流动力学产生不同影响。
