面包酵母腺苷酸环化酶的增溶及其他研究
Solubilization and other studies on adenylate cyclase of baker's yeast.
作者信息
Varimo K, Londesborough J
出版信息
Biochem J. 1976 Nov;159(2):363-70. doi: 10.1042/bj1590363.
Abstract
- Adenylate cyclase of Saccharomyces cerevisiae was sedimented from mechanically disintegrated preparations of yeast over an unusually wide range of centrifugal forces. 2. The enzyme was readily solubilized by Ficoll and by Lubrol PX. Lubrol caused a 2-fold activation. 3. Both particle-bound and Lubrol-solubilized enzyme had an apparent Km for ATP of 1.6 mM in the presence of 0.4 mM-cyclic AMP and 5 mM-MnCl2 at pH 6.2 and 30 degrees C. 4. The Lubrol-solubilized enzyme behaved on gel filtration as a monodisperse protein with an apparent mol.wt. of about 450000.
摘要
- 酿酒酵母的腺苷酸环化酶是从机械破碎的酵母制剂中，在异常广泛的离心力范围内沉降得到的。2. 该酶很容易被聚蔗糖和卢勃罗尔PX溶解。卢勃罗尔引起2倍的激活作用。3. 在pH 6.2和30℃条件下，存在0.4 mM环磷酸腺苷和5 mM氯化锰时，颗粒结合型和卢勃罗尔溶解型酶对ATP的表观Km值均为1.6 mM。4. 卢勃罗尔溶解型酶在凝胶过滤中表现为一种表观分子量约为450000的单分散蛋白质。
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