[关于甲醛和次氯酸钠对采用海涅氏法检测粪便中隐孢子虫卵囊效果的评估]
[The evaluation of the effect of formol and sodium hypochlorite on the demonstration of Cryptosporidium oocysts in feces by Heine's method].
作者信息
Módolo J R, Amato Neto V, Braz L M, Lopes C A, Gottschalk A F
机构信息
Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Universidade Estadual Paulista, SP, Brazil.
出版信息
Rev Soc Bras Med Trop. 1994 Apr-Jun;27(2):75-7. doi: 10.1590/s0037-86821994000200003.
Cryptosporidium oocysts were searched by Heine's method in stools of nine calves with cryptosporidiosis after stool treatment with two disinfectants, 10% paraformaldehyde solution and 14.5% sodium hypochlorite solution. After 30 minutes exposition to sodium hypochlorite solution oocysts became non refractile and acquired a reddish tinge, making their identification difficult. No morphological alterations occurred in oocysts after paraformaldehyde treatment. We recommend paraformaldehyde at 10% concentration as means of human immunodeficiency virus (HIV) inactivation for routine use in stool examinations and therefore making safer those type of procedures for laboratory personnel, when using Heine's method.
采用海涅氏法，在用两种消毒剂（10%多聚甲醛溶液和14.5%次氯酸钠溶液）处理粪便后，对9头患隐孢子虫病的犊牛粪便中的隐孢子虫卵囊进行检测。用次氯酸钠溶液处理30分钟后，卵囊变得不具折光性并呈现微红的色泽，这使得它们的识别变得困难。多聚甲醛处理后卵囊未发生形态改变。我们推荐使用10%浓度的多聚甲醛作为人类免疫缺陷病毒（HIV）灭活剂，以便在粪便检查中常规使用，从而在采用海涅氏法时，让实验室人员在这类操作中更安全。
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