Wells B, Thomson S, Ensor H, Innes E A, Katzer F
Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, EH26 0PZ, United Kingdom.
Biomathematics and Statistics Scotland, James Clerk Maxwell Building, The King's Buildings, Peter Guthrie Tait Road, Edinburgh, EH9 3FD, United Kingdom.
Vet Parasitol. 2016 Aug 30;227:26-9. doi: 10.1016/j.vetpar.2016.07.018. Epub 2016 Jul 16.
Cryptosporidium transmission studies to date have concluded that adult cattle are not a significant source of oocysts contributing to clinical cryptosporidiosis in calves on farm. However current methods of sample processing have been optimised for calf faecal samples and may be less sensitive when used on adult samples due to lower numbers of oocysts and larger size of samples. A modified and novel method of oocyst extraction and concentration was developed and applied in an experiment involving spiking adult cattle faecal samples with known concentrations of Cryptosporidium oocysts. The results showed an increased sensitivity of detection from 100oocysts/g of faecal sample using conventional protocols to 5oocysts/g using the newly developed method. As it is important to be able to accurately assess the contribution of adult ruminants to the transmission of Cryptosporidium, both on farm and in the environment, the development of the techniques described here is likely to make an important contribution to Cryptosporidium transmission studies in future and in subsequent control strategies aimed at the reduction of Cryptosporidium infection in calves on farm.
迄今为止的隐孢子虫传播研究得出结论，成年牛并非导致农场犊牛临床隐孢子虫病的卵囊的重要来源。然而，目前的样本处理方法是针对犊牛粪便样本进行优化的，由于成年样本中的卵囊数量较少且样本体积较大，因此用于成年样本时可能不太敏感。一种经过改进的新型卵囊提取和浓缩方法被开发出来，并应用于一项实验中，该实验涉及用已知浓度的隐孢子虫卵囊对成年牛粪便样本进行加样。结果显示，检测灵敏度从使用传统方法时的每克粪便样本100个卵囊提高到使用新开发方法时的每克5个卵囊。由于能够准确评估成年反刍动物在农场和环境中对隐孢子虫传播的贡献非常重要，这里描述的技术的开发可能会对未来的隐孢子虫传播研究以及随后旨在减少农场犊牛隐孢子虫感染的控制策略做出重要贡献。
