Zeiler T, Riess H, Wittmann G, Hintz G, Zimmermann R, Müller C, Heuft H G, Huhn D
Department of Transfusion Medicine, University Hospital Rudolf Virchow, Free University of Berlin, Germany.
Transfusion. 1994 Aug;34(8):685-9. doi: 10.1046/j.1537-2995.1994.34894353464.x.
Photodynamic virus inactivation of fresh-frozen plasma (FFP) may result in its impaired coagulation capability.
Double-volume plasmapheresis samples from 11 donors were divided in pairs of 250 mL. One group underwent methylene blue (MB) phototreatment (MB-FFP). The other group was treated according to the standards of the American Association of Blood Banks for preparation and storage of FFP. Parameters of hemostasis and clinically important plasma proteins were tested in native plasma, thawed MB-FFP, thawed FFP, and twice-frozen and thawed FFP (FFP-II).
Mean activities of factor V (73.4 vs. 94.5%; p < 0.01), factor VIII (58.1 vs. 86.7%; p < 0.001), and fibrinogen (1.8 vs. 2.8 g/L; p < 0.001) were reduced in MB-FFP as compared to those in FFP. The comparison of MB-FFP to FFP-II revealed reduced activities of factor VIII (58.1 vs. 85.2%; p < 0.001) and fibrinogen (1.8 vs. 2.8 g/L; p < 0.001) but no changes in factor V. Activated partial thromboplastin time in MB-FFP was prolonged beyond the upper normal range (+5.3 sec; p < 0.001) and prothrombin time increased in MB-FFP versus FFP (+0.96 sec; p < 0.001).
MB phototreatment reduces the in vitro coagulation capacity of FFP, most likely as a result of the effects of an additional freezing and thawing procedure and photooxidation-induced protein damage.
新鲜冰冻血浆（FFP）的光动力病毒灭活可能导致其凝血能力受损。
将11名献血者的双倍体积血浆置换样本分成每250 mL一组。一组接受亚甲蓝（MB）光处理（MB - FFP）。另一组按照美国血库协会的标准制备和储存FFP。对天然血浆、解冻后的MB - FFP、解冻后的FFP以及二次冻融的FFP（FFP - II）进行止血参数和临床重要血浆蛋白检测。
与FFP相比，MB - FFP中因子V的平均活性降低（73.4%对94.5%；p < 0.01），因子VIII的平均活性降低（58.1%对86.7%；p < 0.001），纤维蛋白原含量降低（1.8 g/L对2.8 g/L；p < 0.001）。MB - FFP与FFP - II相比，因子VIII活性降低（58.1%对85.2%；p < 0.001），纤维蛋白原含量降低（1.8 g/L对2.8 g/L；p < 0.001），但因子V无变化。MB - FFP的活化部分凝血活酶时间延长至正常范围上限以上（+5.3秒；p < 0.001），与FFP相比，MB - FFP的凝血酶原时间增加（+0.96秒；p < 0.001）。
MB光处理降低了FFP的体外凝血能力，很可能是由于额外的冻融过程和光氧化诱导的蛋白质损伤所致。
