Bode A P, Knupp C L
Department of Pathology, East Carolina University, Greenville, North Carolina.
Transfusion. 1994 Aug;34(8):690-6. doi: 10.1046/j.1537-2995.1994.34894353465.x.
During storage of platelet concentrates at 22 degrees C, changes occur in surface glycoproteins, and membranous vesicles appear in the supernatant plasma. The extent of these changes during refrigerated storage is not known.
Membranous microparticles and changes in surface or total glycoprotein Ib (GPIb) were studied in platelet concentrates divided into aliquots stored at either 4 degrees C or 22 degrees C for 5 days.
The refrigerated platelets showed greater loss of total GPIb, slightly less binding of monoclonal antibodies to surface GPIb, and reduced aggregation response to ristocetin relative to the paired platelet controls at 22 degrees C. Moreover, the platelets stored at 4 degrees C produced 45-percent more microparticles and 64-percent more platelet factor 3 activity in the supernatant plasma than were produced by the controls. These differences were augmented by warming both 4 degrees C- and 22 degrees C-stored platelets at 37 degrees C for 1 to 4 hours.
Storage of platelets at 4 degrees C causes increased membrane vesiculation and accelerated loss of GPIb. The magnitude of these differences was small, but it may contribute to marked reductions in platelet survival in circulation.
在血小板浓缩物于22℃储存期间，表面糖蛋白会发生变化，且膜泡会出现在上清血浆中。冷藏储存期间这些变化的程度尚不清楚。
在分为小份的血小板浓缩物中研究膜微粒以及表面或总糖蛋白Ib（GPIb）的变化，这些小份血小板浓缩物分别在4℃或22℃储存5天。
与在22℃的配对血小板对照相比，冷藏的血小板显示出总GPIb损失更大，单克隆抗体与表面GPIb的结合略少，以及对瑞斯托霉素的聚集反应降低。此外，与对照相比，在4℃储存的血小板在上清血浆中产生的微粒多45%，血小板因子3活性多64%。在37℃将在4℃和22℃储存的血小板温育1至4小时会加剧这些差异。
在4℃储存血小板会导致膜泡形成增加以及GPIb加速丢失。这些差异的幅度较小，但可能导致循环中血小板存活率显著降低。
