Connor J, Currie L M, Allan H, Livesey S A
LifeCell Corporation, Woodlands, Texas, USA.
Transfusion. 1996 Aug;36(8):691-8. doi: 10.1046/j.1537-2995.1996.36896374372.x.
The potential for bacterial contamination limits the storage of platelets at 22 degrees C to 5 days. Refrigerated storage at 4 degrees C would abrogate this problem but would also result in a rapid loss of in vitro viability and functional activity and in vivo viability. The inhibition of platelets during storage by a combination of specific, reversible, second-messenger effectors has been investigated to allow prolonged storage at 4 degrees C with significant retention of in vitro viability and functional activity.
The combination of effectors was added directly to platelet concentrates, and this step was followed by storage at 4 degrees C. Control units were incubated at 4 degrees C without the effectors and at 22 degrees C according to standard blood-banking techniques. At 1, 5, and 9 days, the units were tested for recovery of cell number, recovery of in vitro functional activity and viability, and expression of platelet surface markers.
Treated platelets stored at 4 degrees C for 9 days, while spherical in shape, displayed no loss of cell number and had a recovery of viability and functional activity, as compared with control platelets stored at 22 degrees C for 5 days, as follows: ADP and collagen aggregation responses of 250 and 100 percent, respectively; a 70-percent recovery of hypotonic shock response; and a 60-percent recovery of extent of shape change. The treated platelets also expressed an equivalent amount of the surface marker glycoprotein lb and a lower amount of the activation marker alpha-granule membrane protein-140 on the membrane surface.
Second-messenger effectors added to platelets significantly maintained in vitro functional activity with storage at 4 degrees C. In vitro analysis demonstrates the potential for extended 4 degrees C storage of platelets with numerical and functional recovery comparable to that achieved with current methods. Refrigerated storage of platelet concentrates has the potential to reduce the risk of bacterial contamination.
细菌污染的可能性限制了血小板在22℃下的储存时间为5天。4℃冷藏储存可消除此问题，但也会导致体外活力和功能活性迅速丧失以及体内活力降低。研究了通过特定的、可逆的第二信使效应物组合在储存期间对血小板的抑制作用，以使血小板在4℃下能长时间储存，并显著保留体外活力和功能活性。
将效应物组合直接添加到血小板浓缩物中，然后在4℃下储存。对照单位按照标准血库技术在4℃下不添加效应物进行孵育，并在22℃下孵育。在第1、5和9天，对各单位进行细胞数量恢复、体外功能活性和活力恢复以及血小板表面标志物表达的检测。
在4℃下储存9天的经处理血小板，虽然呈球形，但细胞数量没有损失，与在22℃下储存5天的对照血小板相比，活力和功能活性恢复情况如下：对ADP和胶原的聚集反应分别为250%和100%；低渗休克反应恢复70%；形状变化程度恢复60%。经处理的血小板在膜表面还表达了等量的表面标志物糖蛋白lb和较少量的活化标志物α-颗粒膜蛋白-140。
添加到血小板中的第二信使效应物在4℃储存时能显著维持体外功能活性。体外分析表明，血小板在4℃下延长储存具有潜力，其数量和功能恢复与当前方法相当。血小板浓缩物的冷藏储存有可能降低细菌污染风险。
