Stroncek D F, Ramsey G, Herr G P, Eiber G, Clay M E, Ketyer E C
American Red Cross Blood Services, St. Paul, Minnesota.
Transfusion. 1994 Aug;34(8):706-11. doi: 10.1046/j.1537-2995.1994.34894353468.x.
Antibodies to white cell antigens can cause alloimmune neonatal neutropenia, autoimmune neutropenia, and transfusion reactions.
A full-term male infant developed a skin infection and was found to be neutropenic on his fourth day of life. He had a transient increase in his neutrophil count after treatment with intravenous immunoglobulin, but his neutrophil count was not consistently normal until he was 6 weeks old. Serum from the baby's mother reacted in a granulocyte immunofluorescence assay but not in a granulocyte agglutination assay. The mother's serum was tested in the granulocyte immunofluorescence assay against neutrophils from 103 healthy, unrelated people, and it reacted with cells from 66 percent of those people. The expression of SL correlated weakly with the expression of NA1 (r = 0.23; p = 0.02) and 5a (r = 0.20; p = 0.05) antigens. SL antigen expression on neutrophils was not associated with the expression of NA2, NB1, NB2, NC1, 5b, 9a, or Mart. The expression of SL on neutrophils from members of an extended family was analyzed, and the antigen was found to be inherited in an autosomal-dominant manner. Anti-SL also reacted with T-lymphocytes in a flow cytometry assay but did not react with red cells or platelets. No lymphocytotoxic antibodies were detected in the mother's sera. The anti-SL was tested against neutrophils in an immunoprecipitation and immunoblotting assay, but no molecules were identified. The neutrophil-specific antigens NA are located on Fc gamma receptor III (CD16). To determine if the SL antigen was also located on Fc gamma receptor III, anti-SL was also tested in a monoclonal antibody immobilization of granulocyte antigens assay. Anti-SL did not react with molecules recognized by CD16 monoclonal antibodies.
A new white cell antigen SL, with a frequency of 66 percent, was identified on neutrophils and T-lymphocytes as a result of the evaluation of a case of neonatal alloimmune neutropenia. The molecule bearing the SL antigen was not identified in immunoblotting, immunoprecipitation, or monoclonal antibody immobilization of granulocyte antigens assays.
白细胞抗原抗体可导致同种免疫性新生儿中性粒细胞减少症、自身免疫性中性粒细胞减少症及输血反应。
一名足月儿男婴出现皮肤感染，在出生后第4天被发现中性粒细胞减少。经静脉注射免疫球蛋白治疗后，其中性粒细胞计数短暂升高，但直到6周龄时中性粒细胞计数才持续正常。婴儿母亲的血清在粒细胞免疫荧光试验中有反应，但在粒细胞凝集试验中无反应。用该母亲的血清在粒细胞免疫荧光试验中检测了来自103名健康、无亲缘关系者的中性粒细胞，结果显示血清与其中66%的人的细胞发生反应。SL的表达与NA1（r = 0.23；p = 0.02）和5a（r = 0.20；p = 0.05）抗原的表达呈弱相关。中性粒细胞上SL抗原的表达与NA2、NB1、NB2、NC1、5b、9a或Mart的表达无关。对一个大家庭成员的中性粒细胞上SL的表达进行了分析，发现该抗原以常染色体显性方式遗传。抗SL在流式细胞术试验中也与T淋巴细胞发生反应，但不与红细胞或血小板发生反应。在母亲的血清中未检测到淋巴细胞毒性抗体。在免疫沉淀和免疫印迹试验中用抗SL检测中性粒细胞，但未鉴定出相关分子。中性粒细胞特异性抗原NA位于Fcγ受体III（CD16）上。为确定SL抗原是否也位于Fcγ受体III上，还在粒细胞抗原单克隆抗体固定试验中检测了抗SL。抗SL不与CD16单克隆抗体识别的分子发生反应。
通过对一例新生儿同种免疫性中性粒细胞减少症病例的评估，在中性粒细胞和T淋巴细胞上鉴定出一种新的白细胞抗原SL，其出现频率为66%。在免疫印迹、免疫沉淀或粒细胞抗原单克隆抗体固定试验中均未鉴定出携带SL抗原的分子。
