Stroncek D F, Egging M S, Eiber G A, Clay M E
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
Am J Kidney Dis. 1993 Apr;21(4):368-73. doi: 10.1016/s0272-6386(12)80262-9.
Antibodies to neutrophil cytoplasmic antigens (ANCA) can be detected in patients with Wegener's granulomatosis and systemic vasculitis. During pregnancy or following transfusion, subjects sometimes produce alloantibodies to neutrophil antigens. If patient sera being tested for ANCA contain alloantibodies directed at neutrophil antigens that residue in the cytoplasm, the results may be difficult to interpret. At least one neutrophil antigen, NB1, is expressed on both neutrophil plasma membranes and secondary granules. We tested alloantibodies specific for neutrophil antigens NA1, NA2, NB1, NB2, 5b, 9a, and Mart in an ANCA-indirect immunofluorescence (ANCA-IF) assay to determine if these alloantibodies reacted with neutrophil cytoplasmic or granule antigens. Alloantibodies specific for neutrophil antigens NA1, NA2, NB2, 5b, and 9a did not react with neutrophil cytoplasmic components. However, all three NB1 alloantibodies studied demonstrated a cytoplasmic pattern of immunofluorescence (C-ANCA) when NB1-positive neutrophils were tested. While control ANCA resulted in cytoplasmic immunofluorescence of all neutrophils from each donor tested, NB1 antibodies reacted with a subpopulation of neutrophils from some donors. Cytoplasmic immunofluorescence was also observed with an antibody directed against the Mart neutrophil antigen. The Mart antigen is located on integrin CR3 (CD11b/CD18). To confirm that these reactions were due to anti-Mart, monoclonal antibodies to CD11b and CD18 were also tested and found to cause cytoplasmic immunofluorescence. When the ANCA-IF assay was performed using neutrophils that did not express Mart or NB1 antigen, cytoplasmic immunofluorescence was seen with the ANCA control antisera, but not with the NB1 or Mart alloantibodies.(ABSTRACT TRUNCATED AT 250 WORDS)
在韦格纳肉芽肿病和系统性血管炎患者中可检测到抗中性粒细胞胞浆抗原（ANCA）抗体。在妊娠期间或输血后，受试者有时会产生针对中性粒细胞抗原的同种抗体。如果检测ANCA的患者血清中含有针对残留在细胞质中的中性粒细胞抗原的同种抗体，结果可能难以解释。至少有一种中性粒细胞抗原NB1在中性粒细胞质膜和次级颗粒上均有表达。我们在ANCA间接免疫荧光（ANCA-IF）试验中检测了针对中性粒细胞抗原NA1、NA2、NB1、NB2、5b、9a和Mart的同种抗体，以确定这些同种抗体是否与中性粒细胞胞浆或颗粒抗原发生反应。针对中性粒细胞抗原NA1、NA2、NB2、5b和9a的同种抗体不与中性粒细胞胞浆成分发生反应。然而，在所研究的三种NB1同种抗体中，当检测NB1阳性中性粒细胞时，均显示出胞浆免疫荧光模式（C-ANCA）。虽然对照ANCA导致所检测的每个供体的所有中性粒细胞出现胞浆免疫荧光，但NB1抗体与一些供体的一部分中性粒细胞发生反应。针对Mart中性粒细胞抗原的抗体也观察到胞浆免疫荧光。Mart抗原位于整合素CR3（CD11b/CD18）上。为了证实这些反应是由于抗Mart引起的，还检测了针对CD11b和CD18的单克隆抗体，发现它们会引起胞浆免疫荧光。当使用不表达Mart或NB1抗原的中性粒细胞进行ANCA-IF试验时，ANCA对照抗血清可见胞浆免疫荧光，但NB1或Mart同种抗体则未见。（摘要截短于250字）
