Sica G, Dell'Acqua G, Iacopino F, Fattorossi A, Marchetti P, van der Kwast T H, Pavone-Macaluso M
Istituto di Istologia ed Embriologia Generale, Facoltà di Medicina, Università Cattolica del Sacro Cuore, Rome, Italy.
Urol Res. 1994;22(1):33-8. doi: 10.1007/BF00431546.
Androgen receptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (beta-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with beta-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to beta-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the beta-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.
雄激素受体在细胞系PC - 3中低水平表达，该细胞系对雄激素或抗雄激素均无反应。如果将这些细胞暴露于天然β - 干扰素（β - IFN），通过生化和免疫细胞化学技术评估，会出现细胞生长减少和雄激素受体增加的情况。这种增加似乎与细胞周期任何阶段的PC - 3选择性阻滞无关。用β - IFN预处理可使PC - 3细胞对雄激素双氢睾酮产生部分反应，这表现为细胞数量增加。此外，抗雄激素羟基氟他胺通过增加预先暴露于β - IFN的PC - 3细胞数量而显示出激动特性。当将该抗雄激素与干扰素联合测试时，它会降低β - IFN诱导的细胞生长抑制作用。尚不清楚这些意外效应是由于雄激素受体增加还是其他机制所致。
