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[使用荧光或显色底物的免疫酶分析方法在测定马尔堡病毒抗原中的比较评估]

[A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen].

作者信息

Kutuzov V A, Mikhaĭlov V V, Kruglova S E, Kutuzov V V, Kirillov A P, Krasnianskiĭ V P, Pshenichnov V A, Lebedinskaia E V

出版信息

Vopr Virusol. 1993 Jan-Feb;38(1):17-8.

PMID:8073738
Abstract

A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.

摘要

对使用荧光底物和显色底物的间接固相酶免疫测定法的改进变体进行了比较评估,以测定马尔堡病毒抗原。该方法对前一种底物的分辨能力为3.8×10⁻⁹克蛋白质,对后一种底物为3.1×10⁻⁸克蛋白质。交叉滴定表明与埃博拉病毒不存在共同抗原。

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