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[用于检测埃博拉病毒抗原的免疫酶检测系统的研制]

[Development of the immunoenzyme test-system for detection of Ebola virus antigen].

作者信息

Borisevich I V, Mikhaĭlov V V, Potryvaeva N V, Malinkin Iu N, Kirillov A P, Krasnianskiĭ V P, Markov V I, Makhlaĭ A A, Lebedinskaia E V

出版信息

Vopr Virusol. 1996 Sep-Oct;41(5):232-4.

PMID:8967072
Abstract

An enzyme immunoassay system has been developed for the detection of Ebola virus antigen. It permits a highly accurate and sensitive rapid detection of the antigen. Optimal dilutions of specific immunoglobulin (1:500, corresponding to protein concentration of 50 micrograms/ml) and conjugate were found. The resolving capacity of the new test system is 1.9 x 10(-7) g protein.

摘要

已开发出一种用于检测埃博拉病毒抗原的酶免疫分析系统。它能够高度准确且灵敏地快速检测该抗原。找到了特异性免疫球蛋白(1:500,对应蛋白质浓度为50微克/毫升)和共轭物的最佳稀释度。新测试系统的分辨能力为1.9×10⁻⁷克蛋白质。

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引用本文的文献

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Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus.单克隆抗体的制备以及针对埃博拉病毒包膜糖蛋白GP的抗原捕获酶联免疫吸附测定的开发。
Med Microbiol Immunol. 2004 Nov;193(4):181-7. doi: 10.1007/s00430-003-0204-z. Epub 2003 Oct 31.