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[3H-5尿苷和3H-5亮氨酸在体外培养的细颈涡虫(Iijima)细胞中的掺入]

[Incorporation of 3H-5 uridine and 3H-5 leucine into cultured planarian cells, Polycelis tenuis (Iijima) in vitro].

作者信息

Franquinet R, Lender T, Moigne A L

出版信息

C R Acad Hebd Seances Acad Sci D. 1975 May 21;280(19):2253-6.

PMID:807412
Abstract

RNA and protein synthesis in Planarian cells cultivated in vitro was studied by histoautoradiography. In the non-differentiated cells, uptake of precursor is intense from the beginning of the culture, and sensitive to addition of trophic factor known for their activating effect on mitosis and regeneration. On the contrary the rate of incorporation in differentiated cells is low and uniform, independently of the differents factors added to the medium.

摘要

通过组织放射自显影术研究了体外培养的涡虫细胞中的RNA和蛋白质合成。在未分化细胞中,从培养开始前体摄取就很强烈,并且对已知对有丝分裂和再生具有激活作用的营养因子的添加敏感。相反,分化细胞中的掺入率低且均匀,与添加到培养基中的不同因子无关。

相似文献

1
[Incorporation of 3H-5 uridine and 3H-5 leucine into cultured planarian cells, Polycelis tenuis (Iijima) in vitro].[3H-5尿苷和3H-5亮氨酸在体外培养的细颈涡虫(Iijima)细胞中的掺入]
C R Acad Hebd Seances Acad Sci D. 1975 May 21;280(19):2253-6.
2
[Comparative study of the cells of the freshwater planarian Polycelis tenuis (Iijima) using dissociated fragments cultivated in vitro: ultrastructural aspects and incorporation of (3H) leucine and (3H) uridine].[利用体外培养的解离片段对淡水涡虫Polycelis tenuis(饭岛)细胞进行的比较研究:超微结构方面以及(3H)亮氨酸和(3H)尿苷的掺入]
J Embryol Exp Morphol. 1976 Aug;36(1):41-54.
3
[Action of actinomycin D on the cellular differentiation during regeneration in planarians. II. Autoradiographic histological and ultrastructural studies on the action of antibiotics on RNA synthesis].[放线菌素D对涡虫再生过程中细胞分化的作用。II. 抗生素对RNA合成作用的放射自显影组织学和超微结构研究]
Z Zellforsch Mikrosk Anat. 1971;115(3):442-60.
4
[Demonstration of 2 phases in RNA synthesis during regeneration of planarian (Polycelis tenuis-nigra)].[涡虫(Polycelis tenuis-nigra)再生过程中RNA合成两个阶段的证明]
C R Acad Hebd Seances Acad Sci D. 1976 May 24;282(20):1805-8.
5
[Variations in DNA synthesis and the activities of acid and alkaline deoxyribonucleases during regeneration of planaria (Polycelis tenuis)].[涡虫(细颈多肠涡虫)再生过程中DNA合成及酸性和碱性脱氧核糖核酸酶活性的变化]
C R Seances Acad Sci D. 1980 Jun 30;290(24):1571-4.
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[Evolution of the incorporation of tritiated leucine into the blastema and of the levels of mitosis, during the regeneration of young planarians treated with actinomycin D].[在用放线菌素D处理的幼年涡虫再生过程中,氚标记亮氨酸掺入再生芽基的情况以及有丝分裂水平的变化]
C R Acad Hebd Seances Acad Sci D. 1971 Apr 14;272(15):2017-20.
7
[Effect of actinomycin D and cycloheximide on planarian regeneration after repeated amputation].[放线菌素D和环己酰亚胺对涡虫多次截肢后再生的影响]
C R Acad Hebd Seances Acad Sci D. 1976 Jul 19;283(3):251-4.
8
Effects of serotonin and catecholamines on RNA synthesis in planarians; in vitro and in vivo studies.血清素和儿茶酚胺对涡虫RNA合成的影响;体外和体内研究
Cell Differ. 1981 Aug;10(4):201-9. doi: 10.1016/0045-6039(81)90002-6.
9
Ribonucleic acid metabolism during planarian regeneration.涡虫再生过程中的核糖核酸代谢
Reprod Nutr Dev (1980). 1980;20(5A):1527-37. doi: 10.1051/rnd:19800811.
10
Calcium channel blockers inhibit cellular uptake of thymidine, uridine and leucine: the incorporation of these molecules into DNA, RNA and protein in the presence of calcium channel blockers is not a valid measure of lymphocyte activation.钙通道阻滞剂抑制细胞对胸苷、尿苷和亮氨酸的摄取:在钙通道阻滞剂存在的情况下,将这些分子掺入DNA、RNA和蛋白质中并不是淋巴细胞活化的有效指标。
Immunopharmacology. 1993 Jan-Feb;25(1):75-82. doi: 10.1016/0162-3109(93)90032-l.