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[3H-5尿苷和3H-5亮氨酸在体外培养的细颈涡虫(Iijima)细胞中的掺入]

[Incorporation of 3H-5 uridine and 3H-5 leucine into cultured planarian cells, Polycelis tenuis (Iijima) in vitro].

作者信息

Franquinet R, Lender T, Moigne A L

出版信息

C R Acad Hebd Seances Acad Sci D. 1975 May 21;280(19):2253-6.

PMID:807412
Abstract

RNA and protein synthesis in Planarian cells cultivated in vitro was studied by histoautoradiography. In the non-differentiated cells, uptake of precursor is intense from the beginning of the culture, and sensitive to addition of trophic factor known for their activating effect on mitosis and regeneration. On the contrary the rate of incorporation in differentiated cells is low and uniform, independently of the differents factors added to the medium.

摘要

通过组织放射自显影术研究了体外培养的涡虫细胞中的RNA和蛋白质合成。在未分化细胞中,从培养开始前体摄取就很强烈,并且对已知对有丝分裂和再生具有激活作用的营养因子的添加敏感。相反,分化细胞中的掺入率低且均匀,与添加到培养基中的不同因子无关。

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