Martelly I, Le Moigne A
Reprod Nutr Dev (1980). 1980;20(5A):1527-37. doi: 10.1051/rnd:19800811.
A method for the extraction of total RNA of the planarian Polycelis tenuis is described. This technique has been applied to the study of RNA synthesis in the course of regeneration. Synthesis of RNA begins 7 hrs after sectioning and proceeds in two phases. The first phase, from 7 to 21 hr is characterized by an increased rate of (3H) orotic acid incorporation into RNA up to the 18th after sectioning followed by a decrease between 18 and 21 hrs. The rate of precursor incorporation then had risen again in the second phase to the 30th hr and finally decreased slowly until completion of regeneration. Studies of the pool of nucleotide precursors, show that the observed variations of orotic acid incorporation into RNA reflect variations in RNA synthesis. Electrophoretic analysis of RNA labelled for 2 hrs at various times during regeneration shows that the product formed during the first phase of regeneration is mainly rRNA and low molecular weight RNA. The second phase of the regeneration process, from the 24th hr, is characterized by the appearance of both ribosomal and polydisperse RNAs. These heterogeneous RNA species are detected up to the 46th hr of regeneration, after which time rRNA again becomes predominent. The significance of the two phases of RNA metabolism of which this is the first observation during planarian regeneration is discussed in connection with other biochemical events which have been described during regeneration processes in planarians and other animals.
本文描述了一种提取淡水涡虫Polycelis tenuis总RNA的方法。该技术已应用于再生过程中RNA合成的研究。RNA合成在切片后7小时开始，并分两个阶段进行。第一阶段，从7到21小时，其特征是（3H）乳清酸掺入RNA的速率增加，直至切片后第18小时，随后在18至21小时之间下降。然后在前体掺入速率在第二阶段再次上升至第30小时，最后缓慢下降直至再生完成。对核苷酸前体库的研究表明，观察到的乳清酸掺入RNA的变化反映了RNA合成的变化。对再生过程中不同时间标记2小时的RNA进行电泳分析表明，再生第一阶段形成的产物主要是rRNA和低分子量RNA。再生过程的第二阶段，从第24小时开始，其特征是核糖体RNA和多分散RNA均出现。这些异质RNA种类在再生第46小时之前均可检测到，此后rRNA再次占主导地位。结合涡虫和其他动物再生过程中描述的其他生化事件，讨论了这是涡虫再生过程中首次观察到的RNA代谢两个阶段的意义。
