Xynos F P, Klos D J, Hamilton P D, Schuette V, Huygens P, Fernandez-Pol J A
Laboratory of Molecular Oncology, DVA Medical Center, St. Louis, MO 63106.
Anticancer Res. 1994 May-Jun;14(3A):773-86.
We have identified by cDNA cloning a gene, denoted MPS-1, that is activated in cultured human transformed cells by growth factors. The MPS-1 gene contains one zinc finger domain similar to those present in the C4 family of DNA binding proteins. In this study, the expression of MPS-1 mRNA and protein were examined in HPV-induced human condylomata acuminata. Initially, we detected the presence of MPS-1 mRNA by message amplification phenotyping in all condylomata tissues examined. Subsequently, the cellular distribution and abundance of MPS-1 mRNA was studied by in situ hybridization with specific MPS-1 DNA and RNA probes. We found that MPS-1 mRNA is expressed at high levels in the cytoplasm of condylomata cells. In contrast, the MPS-1 mRNA is expressed at low levels in nonneoplastic tissues. Moreover, antibodies were raised against the predicted N-terminal sequence of the MPS-1 protein and used to detect MPS-1 in condylomata cells. MPS-1 immunoreactivity was detected in the cytoplasm and/or the perinuclear regions of condylomata cells, with marked staining in areas of active proliferation. In distinction, MPS-1 immunoreactivity was very weak in normal epithelial cells. The results support the contention that the MPS-1 protein may be a potentially important mediator of proliferative responses induced by HPV.
我们通过cDNA克隆鉴定出一个名为MPS-1的基因，该基因在培养的人转化细胞中被生长因子激活。MPS-1基因包含一个锌指结构域，类似于DNA结合蛋白C4家族中的那些结构域。在本研究中，我们检测了HPV诱导的人尖锐湿疣中MPS-1 mRNA和蛋白的表达。最初，我们通过信息扩增表型分析在所有检测的湿疣组织中检测到了MPS-1 mRNA的存在。随后，我们用特异性MPS-1 DNA和RNA探针通过原位杂交研究了MPS-1 mRNA的细胞分布和丰度。我们发现MPS-1 mRNA在湿疣细胞的细胞质中高水平表达。相比之下，MPS-1 mRNA在非肿瘤组织中低水平表达。此外，我们制备了针对MPS-1蛋白预测的N端序列的抗体，并用于检测湿疣细胞中的MPS-1。在湿疣细胞的细胞质和/或核周区域检测到MPS-1免疫反应性，在活跃增殖区域有明显染色。相比之下，正常上皮细胞中的MPS-1免疫反应性非常弱。这些结果支持了MPS-1蛋白可能是HPV诱导的增殖反应的潜在重要介质这一论点。
