Ganger D R, Hamilton P D, Fletcher J W, Fernandez-Pol J A
Department of Internal Medicine, Rush Presbyterian St. Luke's Medical Center, Rush Medical College, Chicago, IL 60612, USA.
Anticancer Res. 1997 May-Jun;17(3C):1993-9.
Human metallopanstimulin (MPS-1) is a 9.4-kDa multifunctional ribosomal S27 nuclear "zinc finger" protein which is expressed in a wide variety of actively proliferating cells and tumor tissues. In this paper, we present a case of overexpression of MPS-1 in colon cancer tissues of a seventeen year old male.
Biopsies at the anastomosis and adjacent normal colonic mucosa were obtained by colonoscopy twelve months after right hemicolectomy for an ascending colon well differentiated adenocarcinoma. Immunohistochemical localization of MPS-1 protein was performed by using specific anti-MPS-1 antibodies directed against the N-terminal region of this protein.
Immunohistochemistry demostrated an overexpression of MPS-1 in colonic mucosa crypts in the samples obtained at the anastomosis. In contrast, no expression of MPS-1 was observed in the adjacent normal mucosa. Histopathology performed with hematoxilin and eosin staining revealed focal crypt distortion and proliferation, but no carcinoma.
In this case, the overexpression of MPS-1 was a more definitive evidence of malignant transformation than histology, as demonstrated by the clinical course of the disease. The results support the hypothesis that increased levels of tissue MPS-1 may correlate with a more aggressive behavior of colon malignancy.
人金属全刺激素（MPS - 1）是一种9.4 kDa的多功能核糖体S27核“锌指”蛋白，在多种活跃增殖的细胞和肿瘤组织中表达。在本文中，我们报告了一例17岁男性结肠癌组织中MPS - 1过表达的病例。
在对升结肠高分化腺癌进行右半结肠切除术后12个月，通过结肠镜检查获取吻合口处及相邻正常结肠黏膜的活检组织。使用针对该蛋白N端区域的特异性抗MPS - 1抗体进行MPS - 1蛋白的免疫组化定位。
免疫组化显示，在吻合口处获取的样本中，结肠黏膜隐窝中有MPS - 1过表达。相比之下，在相邻正常黏膜中未观察到MPS - 1表达。苏木精和伊红染色的组织病理学检查显示有局灶性隐窝扭曲和增生，但无癌变。
在该病例中，如疾病的临床病程所示，MPS - 1过表达是比组织学更明确的恶性转化证据。这些结果支持了组织中MPS - 1水平升高可能与结肠恶性肿瘤更具侵袭性的行为相关的假说。
