Sonnewald U, Isern E, Gribbestad I S, Unsgård G
MR-Center, SINTEF UNIMED, Trondheim, Norway.
Anticancer Res. 1994 May-Jun;14(3A):793-8.
NMR spectroscopy was used to analyse perchloric acid extracts of normal human brain, murine brain cell cultures, glioblastoma tissue and the glioblastoma cell line U-87. 1H NMR spectra revealed the presence of elevated levels of UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in glioblastoma extracts and the glioblastoma cell line U-87, in comparison with normal brain tissue and primary cell cultures of neurons and astrocytes. UDP-N-acetylhexosamines appear to accumulate in cells that are unable to differentiate. Furthermore, it was found that the culture medium had an effect on the concentration of UDP-N-acetygalactosamine in the glioblastoma cell line. Hypotaurine, previously only associated with oligodendrocytes, has been identified in astrocyte cultures and in cerebellar granule cells. In normal brain it was not observed by NMR spectroscopy, but was easily detectable in glioblastoma tissue extracts. UDP-N-acetylhexoseamines and hypotaurine might be useful markers for brain pathology and play a role in cell differentiation and cell division.
核磁共振光谱法被用于分析正常人脑、鼠脑细胞培养物、胶质母细胞瘤组织以及胶质母细胞瘤细胞系U - 87的高氯酸提取物。与正常脑组织以及神经元和星形胶质细胞的原代细胞培养物相比，1H核磁共振光谱显示在胶质母细胞瘤提取物和胶质母细胞瘤细胞系U - 87中，UDP - N - 乙酰葡糖胺和UDP - N - 乙酰半乳糖胺水平升高。UDP - N - 乙酰己糖胺似乎在无法分化的细胞中积累。此外，还发现培养基对胶质母细胞瘤细胞系中UDP - N - 乙酰半乳糖胺的浓度有影响。次牛磺酸以前仅与少突胶质细胞有关，现已在星形胶质细胞培养物和小脑颗粒细胞中被鉴定出。在正常脑中，核磁共振光谱未观察到它，但在胶质母细胞瘤组织提取物中很容易检测到。UDP - N - 乙酰己糖胺和次牛磺酸可能是脑病理学的有用标志物，并在细胞分化和细胞分裂中起作用。
