Roth M, Rogers S, Roberts S S, Boyle J M, Müller H J, Weber W
Department of Research, University Hospital Basel, Switzerland.
Anticancer Res. 1994 May-Jun;14(3A):809-15.
We have used a monoclonal antibody specific for UV-induced 6-4 photoproducts in an ELISA assay to determine the kinetics of loss of antigenicity from the DNA of lymphocytes obtained from four groups of people; normal controls and cancer patients who had either received chemotherapy, hormone therapy or no treatment at all. This result was confirmed on a matched pairs analysis of 12 breast cancer patients sampled before and after chemotherapy. We conclude, that chemotherapeutic treatment with alkylating agents modulate the capacity of UV-induced DNA-repair in human lymphocytes in a yet unknown way.
我们在酶联免疫吸附测定（ELISA）中使用了一种对紫外线诱导的6-4光产物具有特异性的单克隆抗体，以确定从四组人群（正常对照组以及接受过化疗、激素治疗或未接受任何治疗的癌症患者）获取的淋巴细胞DNA中抗原性丧失的动力学。在对12名乳腺癌患者化疗前后进行的配对分析中证实了这一结果。我们得出结论，用烷化剂进行的化疗以一种尚不清楚的方式调节人淋巴细胞中紫外线诱导的DNA修复能力。
