Catchpoole D R, Stewart B W
Children's Leukaemia and Cancer Research Centre, University of New South Wales, Prince of Wales Children's Hospital, Sydney, Australia.
Anticancer Res. 1994 May-Jun;14(3A):853-6.
Internucleosomal fragmentation of DNA, the most widely used biochemical indicator of apoptosis, is believed to contribute to loss of viability because the nuclease inhibitor, aurintricarboxylic acid, delays or prevents cell death in a range of experimental systems. We report here that auritricarboxylic acid inhibits topoisomerase II in vitro, the concentration required (< or = 0.2 microM) being less than that usually employed in studies of apoptosis. Since topoisomerase II mediates chromatin condensation during apoptosis, the efficacy of ATA in preventing or delaying cell death may not be the result of nuclease inhibition.
DNA的核小体间断裂是凋亡最广泛使用的生化指标，据信它会导致细胞活力丧失，因为核酸酶抑制剂金精三羧酸在一系列实验系统中可延迟或阻止细胞死亡。我们在此报告，金精三羧酸在体外抑制拓扑异构酶II，所需浓度（≤0.2 microM）低于凋亡研究中通常使用的浓度。由于拓扑异构酶II在凋亡过程中介导染色质浓缩，金精三羧酸预防或延迟细胞死亡的功效可能不是核酸酶抑制的结果。
