Young P, Leeds J M, Slabaugh M B, Mathews C K
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):46-52. doi: 10.1006/bbrc.1994.2146.
Mammalian mitochondria contain pools of deoxyribonucleoside 5'-triphosphates that behave differently from the much larger whole-cell pools. To investigate the origins of these pools, we analyzed HeLa cell mitochondria for ribonucleotide reductase activity. Three findings suggest specific association of a reductase with mitochondria: (1) enzyme activity in extracts of washed mitochondria, (2) stimulation of that activity by dATP at levels inhibitory to the major cellular activity, and (3) association of immunoreactive material with washed and fractionated mitochondria.
哺乳动物线粒体含有脱氧核糖核苷5'-三磷酸池，其行为与大得多的全细胞池不同。为了研究这些池的起源，我们分析了HeLa细胞线粒体的核糖核苷酸还原酶活性。三项发现表明还原酶与线粒体存在特定关联：（1）洗涤过的线粒体提取物中的酶活性，（2）dATP在抑制主要细胞活性的水平下对该活性的刺激，以及（3）免疫反应性物质与洗涤和分级分离的线粒体的关联。
