Loutsch J M, Galvin N J, Bryant M L, Holwerda B C
Department of Pathology, St. Louis University Health Sciences Center, MO 63104.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):472-8. doi: 10.1006/bbrc.1994.2206.
The murine cytomegalovirus UL80 open reading frame was cloned and the predicted amino acid sequence compared with those from other herpesviruses. The open reading frame encodes a fused protease-capsid assembly protein precursor and maintains conserved features including the active site serine, conserved regions CD1 through CD5, the release and maturation sites, and a potential internal cleavage site within the protease. However, the murine cytomegalovirus protease differs in comparison with the other proteases because it contains a unique 15-16 amino acid insertion between CD3 and CD1. The assembly protein sequences are relatively divergent, but they can be arranged into groups defined by herpesvirus subfamily, with each group possessing a conserved motif at its carboxyl terminus.
克隆了鼠巨细胞病毒UL80开放阅读框，并将预测的氨基酸序列与其他疱疹病毒的序列进行了比较。该开放阅读框编码一种融合的蛋白酶-衣壳装配蛋白前体，并保留了保守特征，包括活性位点丝氨酸、CD1至CD5保守区域、释放和成熟位点以及蛋白酶内潜在的内部切割位点。然而，与其他蛋白酶相比，鼠巨细胞病毒蛋白酶有所不同，因为它在CD3和CD1之间包含一个独特的15-16个氨基酸的插入序列。装配蛋白序列相对分散，但可以根据疱疹病毒亚科分为不同的组，每组在其羧基末端具有一个保守基序。
