Rathjen J P, Karageorgos L E, Habili N, Waterhouse P M, Symons R H
Department of Plant Science, Waite Agricultural Research Institute, University of Adelaide, Australia.
Virology. 1994 Feb;198(2):671-9. doi: 10.1006/viro.1994.1079.
Complementary DNAs covering the entire RNA genome of soybean dwarf luteovirus (SDV) were cloned and sequenced. Computer analysis of the 5861 nucleotide sequence revealed five major open reading frames (ORFs) possessing conservation of sequence and organisation with known luteovirus sequences. Comparative analyses of the genome structure show that SDV shares sequence homology and features of gene organisation with barley yellow dwarf virus (PAV isolate) in the 5' half of the genome, yet is more closely related to potato leafroll virus in its 3' coding regions. In addition, SDV differs from other known luteoviruses in possessing an exceptionally long 3' terminal sequence with no apparent coding capacity. We conclude from these data that the SDV genome represents a third variant genome type in the luteovirus group.
克隆并测序了覆盖大豆矮缩黄症病毒（SDV）整个RNA基因组的互补DNA。对5861个核苷酸序列的计算机分析揭示了五个主要的开放阅读框（ORF），它们在序列和组织上与已知的黄症病毒序列具有保守性。基因组结构的比较分析表明，SDV在基因组的5' 半部分与大麦黄矮病毒（PAV分离株）具有序列同源性和基因组织特征，但在其3' 编码区域与马铃薯卷叶病毒关系更密切。此外，SDV与其他已知黄症病毒的不同之处在于，它拥有一个异常长的3' 末端序列，且没有明显的编码能力。从这些数据中我们得出结论，SDV基因组代表了黄症病毒组中的第三种变异基因组类型。
