Interleukin-4 acts as a potent stimulator for expression of monocyte chemoattractant JE/MCP-1 in mouse peritoneal macrophages.

The recruitment of monocyte/macrophages to inflammatory sites is one of the important events in inflammatory reactions. We show herein that interleukin-4 (IL-4) acts as a potent stimulator for expression of monocyte chemoattractant JE/MCP-1 in mouse peritoneal macrophages. IL-4 induced the JE/MCP-1 gene expression in dose and time dependent fashion. Run-on assay suggests that IL-4 stimulates the JE/MCP-1 gene expression at transcriptional level. Monocyte chemotactic activity was detected in culture medium of the cytokine-treated cells. The chemotactic activity in the culture supernatant was completely neutralized by anti-JE/MCP-1 antiserum.