热休克蛋白70RY：热休克蛋白70蛋白家族新成员的进一步特性分析

Hsp70RY: further characterization of a novel member of the hsp70 protein family.

作者信息

Dyer K D, Lavigne M C, Rosenberg H F

机构信息

Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Biochem Biophys Res Commun. 1994 Aug 30;203(1):577-81. doi: 10.1006/bbrc.1994.2221.

DOI:10.1006/bbrc.1994.2221

PMID:8074706

Abstract

Hsp70RY was identified as a member of the hsp70 protein family on the basis of cDNA sequence homology (Fathallah, et. al. (1993) J. Immunol. 151, 810-813). We have shown that mRNA encoding hsp70RY is expressed in a variety of human cell lines and that mRNA expression remains unchanged in human promyelocytic HL-60 cells induced to differentiate with phorbol 12-myristate 13-acetate (PMA). We have also shown that the predicted amino acid sequence of hsp70RY diverges significantly from the other human hsp70 proteins and that it contains a unique glutamate-rich region near its carboxy-terminus. Finally, we have demonstrated the existence of a murine homolog of hsp70RY.

摘要

基于cDNA序列同源性，Hsp70RY被鉴定为热休克蛋白70（hsp70）家族的成员（法塔赫等人，（1993年）《免疫学杂志》151卷，第810 - 813页）。我们已经表明，编码hsp70RY的mRNA在多种人类细胞系中表达，并且在用佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）诱导分化的人类早幼粒细胞HL - 60细胞中，mRNA表达保持不变。我们还表明，hsp70RY的预测氨基酸序列与其他人类hsp70蛋白有显著差异，并且在其羧基末端附近含有一个独特的富含谷氨酸的区域。最后，我们证明了hsp70RY的小鼠同源物的存在。