Ferioli V, Rustichelli C, Vezzalini F, Gamberini G
Dipartimento di Scienze Farmaceutiche, Università di Modena, Italy.
Farmaco. 1994 Jun;49(6):421-5.
This paper reports a RP-HPLC method for the determination in topics of azelaic acid, a keratolytic and anti-comedogenic agent widely used in the treatment of all types of acne. A derivatization step was needed prior to chromatographic analysis because the analyte is lacking in chromophore. A sample clean-up procedure by solid-phase extraction was also developed to analyse azelaic acid in complex matrices, such as pharmaceutical and cosmetic formulations.
本文报道了一种反相高效液相色谱法，用于测定壬二酸，壬二酸是一种角质剥脱剂和抗粉刺剂，广泛用于治疗各类痤疮。由于分析物缺乏发色团，在色谱分析之前需要进行衍生化步骤。还开发了一种通过固相萃取的样品净化程序，用于分析复杂基质中的壬二酸，如药物制剂和化妆品配方。
