Gatti R, Andrisano V, Di Pietra A M, Cavrini V
Department of Pharmaceutical Sciences, University of Bologna, Italy.
J Pharm Biomed Anal. 1995 Apr;13(4-5):589-95. doi: 10.1016/0731-7085(95)01305-5.
2-Bromoacetyl-6-methoxynaphthalene has been found to be a useful prechromatographic fluorescent labelling reagent for the analysis of dicarboxylic acids. The derivatization reaction of azelaic acid and meglutol with this reagent yieded stable and highly fluorescent diesters which could be analysed by reversed-phase HPLC with fluorescence detection. According to the nature of the sample, the derivatization reaction could be carried out in acetonitrile or in an aqueous micellar system. The proposed methods proved to be suitable for the quality control of various complex pharmaceutical and cosmetic formulations of the azelaic acid and meglutol.
已发现2-溴乙酰基-6-甲氧基萘是一种用于分析二羧酸的有用的色谱前荧光标记试剂。壬二酸和葡烟醇与该试剂的衍生化反应产生了稳定且高荧光的二酯，这些二酯可通过反相高效液相色谱结合荧光检测进行分析。根据样品的性质，衍生化反应可在乙腈或水性胶束体系中进行。所提出的方法被证明适用于各种含壬二酸和葡烟醇的复杂药物和化妆品制剂的质量控制。
