Sauer B
DuPont Merck Pharmaceutical Co., Wilmington, DE.
Biotechniques. 1994 Jun;16(6):1086-8.
A series of excisable marker cassettes has been constructed to facilitate recycling of selectable markers in yeast. These cassettes exploit the use of the Cre DNA recombinase to precisely excise the marker gene when desired. They are especially useful for making gene disruptions and then removing the marker gene to allow subsequent genetic manipulations with that same marker. Also described are a number of cre expression vectors that allow galactose-induced expression of the recombinase in yeast. The procedure is simple and allows rapid processing of large numbers of transformants.
已经构建了一系列可切除的标记盒，以促进酵母中选择标记的循环利用。这些盒利用Cre DNA重组酶在需要时精确切除标记基因。它们对于进行基因破坏，然后去除标记基因以允许使用同一标记进行后续遗传操作特别有用。还描述了许多cre表达载体，这些载体允许在酵母中半乳糖诱导重组酶的表达。该方法简单，可快速处理大量转化体。
