Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.
Methods Mol Biol. 2022;2489:369-393. doi: 10.1007/978-1-0716-2273-5_19.
Plant natural products (PNPs) are valuable resources for the development of pharmaceuticals and agrochemicals, yet the biosynthesis and metabolism of PNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pathways. Here, we describe the reconstitution and verification of the upstream part of brassinolide biosynthesis in S. cerevisiae using this method.
植物天然产物 (PNPs) 是开发药物和农用化学品的宝贵资源，但 PNP 的生物合成和代谢在很大程度上仍是未知的。异源途径重建是次生代谢产物特征描述的一种广泛采用的策略。酵母系统已广泛应用于 PNP 的异源生产，被认为是研究植物生物合成途径的有前途的平台。在这里，我们使用该方法描述了油菜素内酯生物合成上游部分在酿酒酵母中的重建和验证。
