Shirakura T, Hashimoto T, Nakamura Y, Kamaishi T, Cao Y, Adachi J, Hasegawa M, Yamamoto A, Goto N
Department of Oral Microbiology, School of Dentistry, Showa University, Tokyo, Japan.
Jpn J Genet. 1994 Apr;69(2):119-35. doi: 10.1266/jjg.69.119.
Partial DNA regions encoding a major part of translation elongation factor 2 (EF-2) from a mitochondria-lacking protozoan, Entamoeba histolytica, were amplified by polymerase chain reaction and their primary structures were analyzed. The deduced amino acid sequence was aligned with other eukaryotic and archaebacterial EF-2's, and the phylogenetic relationships among eukaryotes were inferred by the maximum likelihood (ML) method. The ML analyses using four different stochastic models of amino acid substitutions consistently suggest that among eukaryotic species being analyzed, E. histolytica is likely to have diverged from other higher eukaryotes on the early phase of eukaryotic evolution.
通过聚合酶链反应扩增了来自缺乏线粒体的原生动物溶组织内阿米巴的编码翻译延伸因子2（EF-2）主要部分的部分DNA区域，并分析了它们的一级结构。将推导的氨基酸序列与其他真核生物和古细菌的EF-2进行比对，并通过最大似然（ML）方法推断真核生物之间的系统发育关系。使用四种不同氨基酸替换随机模型的ML分析一致表明，在所分析的真核生物物种中，溶组织内阿米巴可能在真核生物进化的早期阶段就与其他高等真核生物发生了分化。
