Mizuta K, Hashimoto T, Otaka E
Division of Molecular Biology, Hiroshima University, Japan.
Curr Genet. 1995 Jun;28(1):19-25. doi: 10.1007/BF00311877.
We previously reported the sequence of YL8A, one of the two genes encoding yeast ribosomal protein YL8. With the aim of conducting an evolutionary study we have cloned and sequenced a second gene, YL8B. The disruption of both genes is lethal. Unlike other duplicated ribosomal protein genes, each open reading frame is interrupted by two introns containing long conserved sequences. A comparison of nucleotide and amino-acid sequences reveals that the duplication of the YL8 gene must have occurred very recently. Alignment and phylogenetic analysis of the amino-acid sequences of YL8-related proteins from various species show the existence not only of YL8 ribosomal proteins but also of a family of YL8-like proteins. These are present in at least three species of yeast and seem to be functionally distinct from ribosomal proteins.
我们之前报道了编码酵母核糖体蛋白YL8的两个基因之一YL8A的序列。为了进行进化研究，我们克隆并测序了第二个基因YL8B。两个基因的破坏都是致死性的。与其他重复的核糖体蛋白基因不同，每个开放阅读框都被两个含有长保守序列的内含子打断。核苷酸和氨基酸序列的比较表明，YL8基因的复制肯定是最近才发生的。对来自不同物种的与YL8相关蛋白的氨基酸序列进行比对和系统发育分析，结果显示不仅存在YL8核糖体蛋白，还存在一个类似YL8的蛋白家族。这些蛋白至少存在于三种酵母中，并且在功能上似乎与核糖体蛋白不同。
