Kaneko T, Wada H, Wakita Y, Minamikawa K, Nakase T, Mori Y, Deguchi K, Shirakawa S
Second Department of Internal Medicine, Mie University School of Medicine, Japan.
Blood Coagul Fibrinolysis. 1994 Jun;5(3):385-92.
The expression of tissue factor (TF) and plasminogen activator inhibitor (PAI)-1 was induced in cultured human umbilical vein cells (HUVEC) by very low density lipoprotein (VLDL). VLDL had a strong capacity for augmenting the expression of TF and PAI-1, while the capacity of LDL or high density lipoprotein (HDL) in this regard was very weak. VLDL and LDL also elevated TF activity in monocyte culture medium (VLDL, LDL) and the conditioned medium markedly elevated TF and PAI-1 production in HUVEC compared with directly added lipoproteins. These findings indicated that lipoproteins affect both monocyte-macrophages and endothelial cells, and that they cause a hypercoagulable-hypofibrinolytic state. It is thus possible that hyperlipidaemia could be a direct risk factor for thrombotic disease even if atherosclerotic lesions are not present.
极低密度脂蛋白（VLDL）可诱导培养的人脐静脉细胞（HUVEC）中组织因子（TF）和纤溶酶原激活物抑制剂（PAI）-1的表达。VLDL增强TF和PAI-1表达的能力很强，而低密度脂蛋白（LDL）或高密度脂蛋白（HDL）在这方面的能力则很弱。VLDL和LDL还可提高单核细胞培养基中的TF活性（VLDL、LDL），与直接添加脂蛋白相比，条件培养基显著提高了HUVEC中TF和PAI-1的产生。这些发现表明，脂蛋白会影响单核细胞-巨噬细胞和内皮细胞，并导致高凝-低纤溶状态。因此，即使不存在动脉粥样硬化病变，高脂血症也可能是血栓形成疾病的直接危险因素。
