利钠肽调节内皮细胞中组织因子和纤溶酶原激活物抑制剂-1的表达。
Natriuretic peptides regulate the expression of tissue factor and PAI-1 in endothelial cells.
作者信息
Yoshizumi M, Tsuji H, Nishimura H, Masuda H, Kunieda Y, Kawano H, Kimura S, Sugano T, Kitamura H, Nakagawa K, Nakagawa M
机构信息
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
出版信息
Thromb Haemost. 1999 Nov;82(5):1497-503.
In the present study, we demonstrate that brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) interact with angiotensin II (Ang II) in regulative blood coagulation and fibrinolysis by suppressing the expressions of both tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) induced by Ang II. The expressions of TF and PAI-1 mRNA were analyzed by northern blotting methods, and the activities of TF on the surface of rat aortic endothelial cells (RAECs) and PAI-1 in the culture media were respectively measured by chromogenic assay. Both BNP and CNP suppressed the expressions of TF and PAI-1 mRNA induced by Ang II in a time- and concentration-dependent manner via cGMP cascade, which suppressions were accompanied by respective decrease in activities of TF and PAI-1. However, neither the expression of tissue factor pathway inhibitor (TFPI) nor tissue-type plasminogen activator (TPA) mRNA was affected by the treatment of BNP and CNP.
在本研究中，我们证明脑钠肽（BNP）和C型钠肽（CNP）通过抑制血管紧张素II（Ang II）诱导的组织因子（TF）和纤溶酶原激活物抑制剂-1（PAI-1）的表达，在调节血液凝固和纤维蛋白溶解过程中与Ang II相互作用。采用Northern印迹法分析TF和PAI-1 mRNA的表达，通过显色法分别测定大鼠主动脉内皮细胞（RAECs）表面TF的活性和培养基中PAI-1的活性。BNP和CNP均通过cGMP级联反应以时间和浓度依赖性方式抑制Ang II诱导的TF和PAI-1 mRNA的表达，这种抑制伴随着TF和PAI-1活性的相应降低。然而，BNP和CNP处理对组织因子途径抑制剂（TFPI）的表达以及组织型纤溶酶原激活物（TPA）mRNA均无影响。
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