Barker H, Webster K D, Jolly C A, Reavy B, Kumar A, Mayo M A
Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom.
Mol Plant Microbe Interact. 1994 Jul-Aug;7(4):528-30. doi: 10.1094/mpmi-7-0528.
Four potato clones with host gene-mediated resistance to potato leafroll virus (PLRV) multiplication were transformed with the PLRV coat protein (CP) gene. Plants of lines expressing high levels of transcript were highly resistant to PLRV multiplication; virus concentration was only 20-40 ng/g of leaf, which is approximately 1% of the concentration reached in susceptible cultivars. The effects of the transgenic and host-derived resistance genes appear to be additive.
将4个具有宿主基因介导的抗马铃薯卷叶病毒(PLRV)增殖能力的马铃薯克隆株系用PLRV外壳蛋白(CP)基因进行转化。表达高水平转录本的株系植株对PLRV增殖具有高度抗性;病毒浓度仅为20 - 40 ng/g叶片,约为感病品种中所达到浓度的1%。转基因抗性基因和宿主衍生抗性基因的作用似乎具有累加性。