通过结合宿主基因和转基因的效应增强马铃薯对马铃薯卷叶病毒增殖的抗性。
Enhancement of resistance to potato leafroll virus multiplication in potato by combining the effects of host genes and transgenes.
作者信息
Barker H, Webster K D, Jolly C A, Reavy B, Kumar A, Mayo M A
机构信息
Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom.
出版信息
Mol Plant Microbe Interact. 1994 Jul-Aug;7(4):528-30. doi: 10.1094/mpmi-7-0528.
Four potato clones with host gene-mediated resistance to potato leafroll virus (PLRV) multiplication were transformed with the PLRV coat protein (CP) gene. Plants of lines expressing high levels of transcript were highly resistant to PLRV multiplication; virus concentration was only 20-40 ng/g of leaf, which is approximately 1% of the concentration reached in susceptible cultivars. The effects of the transgenic and host-derived resistance genes appear to be additive.
将4个具有宿主基因介导的抗马铃薯卷叶病毒（PLRV）增殖能力的马铃薯克隆株系用PLRV外壳蛋白（CP）基因进行转化。表达高水平转录本的株系植株对PLRV增殖具有高度抗性；病毒浓度仅为20 - 40 ng/g叶片，约为感病品种中所达到浓度的1%。转基因抗性基因和宿主衍生抗性基因的作用似乎具有累加性。
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