Moore J M, Casale J F
United States Drug Enforcement Administration, Special Testing and Research Laboratory, McLean, VA 22102-3494.
J Chromatogr A. 1994 Jul 15;674(1-2):165-205. doi: 10.1016/0021-9673(94)85224-3.
Chromatographic methodology used for the in-depth alkaloid analyses of coca leaves and for the characterization of alkaloidal impurities and manufacturing by-products in illicit refined cocaine samples is reviewed. This includes liquid-liquid partition and liquid-solid adsorption column chromatography, packed- and capillary-column gas chromatography with flame-ionization, electron-capture, nitrogen-phosphorous and mass spectrometric detection, and high-performance liquid chromatography with ultraviolet detection. The rationale supporting the presence and determination of processing impurities/by-products in cocaine samples is discussed, and chromatographic methodology used for the development of drug impurity signature profiles is presented.
本文综述了用于古柯叶生物碱深度分析以及非法精制可卡因样品中生物碱杂质和制造副产物表征的色谱方法。这包括液-液分配和液-固吸附柱色谱法、配备火焰离子化、电子捕获、氮磷和质谱检测的填充柱和毛细管柱气相色谱法，以及配备紫外检测的高效液相色谱法。文中讨论了支持可卡因样品中加工杂质/副产物存在及测定的基本原理，并介绍了用于建立药物杂质特征图谱的色谱方法。
