Shambaugh G E, Wilber J F, Montoya E, Ruder H, Blonsky E R
J Clin Endocrinol Metab. 1975 Jul;41(1):131-4. doi: 10.1210/jcem-41-1-131.
Levels of thyrotropin-releasing hormone (TRH) were quantitated in human lumbar spinal fluid (CSF) utilizing a sensitive and specific TRH radioimmunoassay. Endogenous TRH was sufficiently stable in CSF to permit 85% recovery of intact TRH after 48 h storage at 4 C. TRH levels in AM or PM samples obtained from 15 women and 12 men were easily detected in all CSF specimens. No significant difference between the TRH concentration in CSF of men and women was observed (44.2+/-6.8 and 38.1+/-6.5 pg/ml (mean+/-SE) respectively). TRH concentrations were 40.2+/-6.9 pg/ml (mean+/-SEM) in AM and 41.4+/-8.0 pg/ml in PM samples. By contrast, CSF cortisol levels obtained concurrently were twofold higher in AM than PM (0.68+/-0.08 vs 0.38+/-0.02 mug/100 ml (mean+/-SEM) respectively, P less than 0.001). These data are consistent with the possibility that a portion of the TRH in CSF can be derived from the central nervous system (CNS) and unrelated to the hypo-physiotropic control of thyrotropin (TSH) synthesis and secretion.
利用一种灵敏且特异的促甲状腺激素释放激素（TRH）放射免疫分析法对人腰椎脑脊液（CSF）中的TRH水平进行了定量分析。内源性TRH在脑脊液中足够稳定，在4℃储存48小时后，完整TRH的回收率可达85%。在所有脑脊液标本中均能轻松检测到从15名女性和12名男性获取的上午或下午样本中的TRH水平。未观察到男性和女性脑脊液中TRH浓度存在显著差异（分别为44.2±6.8和38.1±6.5 pg/ml（平均值±标准误））。上午样本中的TRH浓度为40.2±6.9 pg/ml（平均值±标准误），下午样本中的TRH浓度为41.4±8.0 pg/ml。相比之下，同时测得的脑脊液皮质醇水平上午比下午高出两倍（分别为0.68±0.08与0.38±0.02 μg/100 ml（平均值±标准误），P<0.001）。这些数据支持以下可能性：脑脊液中的一部分TRH可能源自中枢神经系统（CNS），且与促甲状腺激素（TSH）合成和分泌的下丘脑-垂体控制无关。
