用于分离流产布鲁氏菌的液体和固体培养基。
Fluid and solid media for isolation of Brucella abortus.
作者信息
Brodie J, Sinton G P
出版信息
J Hyg (Lond). 1975 Jun;74(3):359-67. doi: 10.1017/s0022172400046878.
The use of a fluid-enrichment technique increased by 10-16% the recovery rate of Brucella abortus from milks of herds positive to a milk ring test. Three solid media for direct cultural techniques were also tested on the same milks. Combinations of these techniques produced a recovery rate of ca. 88% from MRT-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the MRT cream layer as inoculum. B. abortus biotypes 1-5 and 9 were isolated and growth of all the biotypes was supported by all media used.
采用液体富集技术后，从乳环试验呈阳性的畜群牛奶中分离出流产布鲁氏菌的回收率提高了10%-16%。还在相同的牛奶上测试了三种用于直接培养技术的固体培养基。这些技术的组合使来自乳环试验呈阳性畜群牛奶的回收率约为88%，采用的接种物是离心沉淀物和乳脂层的混合物，而以乳环试验乳脂层作为接种物时的回收率约为75%。分离出了流产布鲁氏菌生物型1-5和9，所有使用的培养基均能支持所有生物型的生长。
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