Bradley D E, Pitt T L
J Hyg (Lond). 1975 Jun;74(3):419-30. doi: 10.1017/s0022172400046933.
An attempt was made to correlate serological relationships determined by the pili, the flagella and the O-somatic antigens of Pseudomonas aeruginosa, and to make a preliminary assessment of use of the pilus antigen as an epidemiological marker. A method is described for the preparation of antiserum specific for the "normal' PSA pili of P. aeruginosa. A high titre of pilus antibodies was obtained by immunizing rabbits with mutants whose pili had lost their ability to retract into the cell. The "normal' form of the organism, with retractile pili, was poorly agglutinated by high-titre anti-pilus serum, but suspensions of it that had been treated with osmium tetroxide showed greatly increased agglutinability. Antibody labelling for electron microscopy was used to determine the serological relations of pili and of flagella for P. aeruginosa strains belonging to different serological groups as defined by O-somatic antigens. The distribution of pilar and flagellar antigens among strains was not correlated with the O-somatic serotype. A strain of P. aeruginosa carrying a drug-resistance plasmid had fewer "normal' PSA pili than the background strain.
本研究旨在关联由铜绿假单胞菌的菌毛、鞭毛和O-菌体抗原所确定的血清学关系，并对菌毛抗原作为流行病学标志物的用途进行初步评估。本文描述了一种制备针对铜绿假单胞菌“正常”PSA菌毛的特异性抗血清的方法。通过用菌毛失去缩回细胞能力的突变体免疫兔子，获得了高滴度的菌毛抗体。具有可缩回菌毛的该生物体“正常”形式，被高滴度抗菌毛血清凝集性较差，但经四氧化锇处理后的菌悬液凝集性大大增加。利用电子显微镜抗体标记法来确定属于由O-菌体抗原定义的不同血清学组的铜绿假单胞菌菌株的菌毛和鞭毛的血清学关系。菌株间菌毛和鞭毛抗原的分布与O-菌体血清型无关。携带耐药质粒的铜绿假单胞菌菌株比背景菌株的“正常”PSA菌毛更少。
